PMID- 8118097
OWN - NLM
STAT- MEDLINE
DCOM- 19940401
LR  - 20190904
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 4
IP  - 9
DP  - 1993 Sep
TI  - In situ analysis of centromere segregation in C57BL/6 x Mus spretus interspecific 
      backcrosses.
PG  - 475-80
AB  - The analysis of major satellite sequence differences between Mus spretus and 
      laboratory mice provides a robust method for analyzing the centromere location 
      for the genetic maps of each mouse chromosome. Fluorescence in situ hybridization 
      (FISH) of a genomic probe, pMR196, for the laboratory mouse major satellite 
      sequences was used to identify C57BL/6Ros (B6) pericentromeric heterochromatin in 
      progeny of reciprocal backcross matings. These included 80 (B6 x M. spretus)F1 x 
      M. spretus progeny (BSS) and 70 (B6 x M. spretus)F1 x B6 (BSB) progeny. FISH 
      analysis of pericentromeric heterochromatin was conducted on the same metaphase 
      spreads that were karyotypically analyzed for chromosome-specific banding 
      patterns. Analysis of chromosomal segregation suggested that there was not 
      primary deviation from random assortment during meiosis in the interspecific 
      hybrid female, because nearly all of the 190 pair-wise comparisons did not 
      deviate from expected and because there was no consistent pattern of deviation of 
      the same chromosomes in the reciprocal backcross progeny from similar (C57BL/6 x 
      M. spretus)F1 hybrid females. These results affirm the value of using the major 
      satellite to genetically mark pericentromeric heterochromatin in the analysis of 
      the segregation and assortment of centromeres in Mus interspecific crosses.
FAU - Matsuda, Y
AU  - Matsuda Y
AD  - Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, 
      Buffalo, New York 14263.
FAU - Manly, K F
AU  - Manly KF
FAU - Chapman, V M
AU  - Chapman VM
LA  - eng
GR  - HG-00170/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Animals
MH  - *Centromere
MH  - Chromosomes
MH  - Crosses, Genetic
MH  - DNA, Satellite
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL/*genetics
MH  - Muridae/*genetics
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1007/BF00364780 [doi]
PST - ppublish
SO  - Mamm Genome. 1993 Sep;4(9):475-80. doi: 10.1007/BF00364780.