PMID- 8118867
OWN - NLM
STAT- MEDLINE
DCOM- 19940401
LR  - 20161123
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 153
IP  - 2
DP  - 1994 Feb
TI  - Alteration of tumor necrosis factor-alpha production by macrophages from mice fed 
      diets high in eicosapentaenoic and docosahexaenoic fatty acids.
PG  - 287-97
AB  - Dietary exposure to n-3 fats found in marine fish oils are known to reduce 
      certain inflammatory conditions. Although depressed prostaglandin E2 (PGE2) 
      production is thought to be a major mechanism of the beneficial effects, the 
      direct effects of n-3 fatty acids on inflammatory macrophage function are not 
      well understood. In this study, production of the inflammatory monokine, tumor 
      necrosis factor-alpha (TNF alpha), by isolated murine macrophages was assessed 
      following a 3-week feeding with diets containing either 10% menhaden fish oil as 
      a source of n-3 fatty acids or, as a control and source of n-6 fatty acids, 10% 
      safflower oil. Cultures of peritoneal macrophages from mice fed diets with n-3 
      fatty acids had more TNF alpha activity 24 hr after in vitro stimulation with 
      bacterial lipopolysaccharide than did macrophages from mice fed the 
      n-6-containing diet. The onset and maximal synthesis of bioactive TNF alpha and 
      down-regulation of messenger RNA for TNF alpha appeared to be similar for the two 
      diets, suggesting that macrophages from mice fed a diet high in n-6 but not n-3 
      fatty acids were capable of removing active TNF alpha from culture media. 
      Experiments in which PGE2 was added exogenously indicated that the removal of TNF 
      alpha from culture supernatant by macrophages was induced by lower concentrations 
      of PGE2 than that associated with termination of production, and that n-3 fatty 
      acid diets caused a selective loss in the clearance mechanism. These results 
      demonstrate a specific alteration of PGE2-mediated regulation of 
      macrophage-produced TNF alpha by n-3 fatty acids.
FAU - Somers, S D
AU  - Somers SD
AD  - Division of Immunology, James N. Gamble Institute of Medical Research, 
      Cincinnati, Ohio 45319.
FAU - Erickson, K L
AU  - Erickson KL
LA  - eng
GR  - R01 CA 47050/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Dietary Fats, Unsaturated)
RN  - 0 (Fish Oils)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1D8HWC57D0 (Menhaden oil)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Dietary Fats, Unsaturated/pharmacology
MH  - Dinoprostone/metabolism/pharmacology
MH  - Docosahexaenoic Acids/*pharmacology
MH  - Eicosapentaenoic Acid/*pharmacology
MH  - Female
MH  - Fish Oils/pharmacology
MH  - In Vitro Techniques
MH  - Indomethacin/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages, Peritoneal/*drug effects/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - S0008-8749(84)71029-X [pii]
AID - 10.1006/cimm.1994.1029 [doi]
PST - ppublish
SO  - Cell Immunol. 1994 Feb;153(2):287-97. doi: 10.1006/cimm.1994.1029.