PMID- 8124932
OWN - NLM
STAT- MEDLINE
DCOM- 19940411
LR  - 20191101
IS  - 0739-7240 (Print)
IS  - 0739-7240 (Linking)
VI  - 11
IP  - 1
DP  - 1994 Jan
TI  - Cumulus and oocyte maturation and in vitro and in vivo fertilization of oocytes 
      in relation to follicular steroids, prolactin, and glycosaminoglycans throughout 
      the estrous period in superovulated heifers with a normal LH surge, no detectable 
      LH surge, and progestin inhibition of LH surge.
PG  - 59-86
AB  - Crossbred heifers (n = 103) were synchronized to estrus with prostaglandin (PGF2 
      alpha) and superovulated with follicle stimulating hormone (FSH-P). Animals were 
      ovariectomized every 12 hr after the PGF2 alpha injection (n = 7 to 9/time) up to 
      108 hr to monitor the follicular, hormonal, and oocyte changes associated with 
      follicular development and ovulation. Twenty-eight animals were implanted with 
      Norgestomet implants 12 hr before PGF2 alpha and ovariectomized at 72, 84, 96, 
      and 108 hr post PGF2 alpha injection to monitor effects of progesterone and 
      suppression of the luteinizing hormone (LH) surge on oocyte maturation and 
      quality. Follicular fluid was collected and analyzed for progesterone, estradiol, 
      prolactin, and glycosaminoglycan content in conjunction with cumulus maturation 
      and nuclear stage of oocyte maturation. Analysis of in vivo matured oocytes by in 
      vitro fertilization was carried out at 60, 72, 84, and 96 hr post PGF2 alpha and 
      in vitro matured oocytes at 12 to 108 hr post PGF2 alpha. No developmental 
      changes in cumulus cells surrounding the oocyte of small follicles was noted (< 
      or = 4 mm dia) indicating a static population. Medium (> 4 < or = 8 mm) and large 
      size (> 8 mm) follicles developed to the corona radiata and loose cumulus stages 
      in animals in which an LH surge was detected but cumulus status remained 
      primarily in the tight cumulus stage for animals without an LH surge. The 
      estradiol-to-progesterone ratio for tight cumulus (TC), corona radiata (CR), and 
      loose cumulus (LC) stages was 1.8 +/- .1, 1.0 +/- .1, and .4 +/- .2, respectively 
      (P < .01). Nuclear maturation of oocytes in small follicles from animals without 
      a detectable LH surge seem to indicate early maturation (48 to 72 hr post PGF2 
      alpha) in conjunction with a high percent of degenerate oocytes not seen in 
      animals exhibiting an LH surge. Oocytes from medium size follicles matured to 
      germinal vesicle breakdown (GVBD) and early meiosis (metaphase I; MI) stages of 
      development in all treatments. Most oocytes were degenerate in 
      Norgestomet-implanted animals. Oocytes from large follicles (> 8 mm dia) from 
      animals exhibiting an LH surge were in MI and metaphase II (MII) stages (48 to 84 
      hr post PGF2 alpha) in preparation of ovulation whereas oocytes from animals not 
      exhibiting an LH surge had oocytes that early matured to MII (48 to 72 hr post 
      PGF2 alpha), later regressing to degenerate oocytes (84 to 108 hr). Follicular 
      progesterone, estradiol, and prolactin increased with oocyte maturation, 
      particularly in medium and large follicles.(ABSTRACT TRUNCATED AT 400 WORDS)
FAU - Wise, T
AU  - Wise T
AD  - USDA, Agricultural Research Service, Clay Center, Nebraska 68933-0166.
FAU - Suss, U
AU  - Suss U
FAU - Stranzinger, G
AU  - Stranzinger G
FAU - Wuthrich, K
AU  - Wuthrich K
FAU - Maurer, R R
AU  - Maurer RR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Glycosaminoglycans)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-62-4 (Prolactin)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Cattle/*physiology
MH  - Estradiol/chemistry
MH  - Estrus/*physiology
MH  - Female
MH  - *Fertilization in Vitro
MH  - Follicular Fluid/*chemistry
MH  - Glycosaminoglycans/*chemistry/physiology
MH  - Luteinizing Hormone/antagonists & inhibitors/*metabolism
MH  - Oocytes/*physiology
MH  - Ovarian Follicle/physiology
MH  - Progesterone/chemistry/*pharmacology
MH  - Prolactin/*chemistry/physiology
MH  - Superovulation/*physiology
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 0739-7240(94)90036-1 [pii]
AID - 10.1016/0739-7240(94)90036-1 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 1994 Jan;11(1):59-86. doi: 10.1016/0739-7240(94)90036-1.