PMID- 8125011
OWN - NLM
STAT- MEDLINE
DCOM- 19940414
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 66
IP  - 3
DP  - 1994
TI  - Fast, sensitive multicolor detection of nucleic acids in situ by PRimed IN Situ 
      labeling (PRINS).
PG  - 152-4
AB  - PRimed IN Situ labeling (PRINS) has become an alternative to traditional 
      fluorescence in situ hybridization (FISH) methods for detection of nucleic acids 
      in situ. PRINS is based on sequence-specific annealing in situ of an unlabeled 
      DNA probe. The probe serves as a primer for chain elongation in situ, catalyzed 
      by a suitable DNA polymerase that uses labeled nucleotides as substrate. The fact 
      that the probe is unlabeled means that high probe concentrations can be utilized, 
      making the hybridization very fast. We describe here a fast method for detection 
      of three different target sequences visualized in different colors with PRINS. An 
      advantage, relative to FISH, is that even probes with different melting 
      temperatures can be detected in the same metaphase with optimal stringency for 
      each probe.
FAU - Hindkjaer, J
AU  - Hindkjaer J
AD  - Institute of Human Genetics, University of Aarhus, Denmark.
FAU - Koch, J
AU  - Koch J
FAU - Terkelsen, C
AU  - Terkelsen C
FAU - Brandt, C A
AU  - Brandt CA
FAU - Kolvraa, S
AU  - Kolvraa S
FAU - Bolund, L
AU  - Bolund L
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (Nucleic Acids)
SB  - IM
MH  - DNA Probes
MH  - Humans
MH  - Nucleic Acid Hybridization/*methods
MH  - Nucleic Acids/*analysis
MH  - X Chromosome
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1159/000133688 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1994;66(3):152-4. doi: 10.1159/000133688.