PMID- 8125753
OWN - NLM
STAT- MEDLINE
DCOM- 19940412
LR  - 20100324
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 35
IP  - 3
DP  - 1994 Mar
TI  - Cytokines in vitreous humor: interleukin-6 is elevated in proliferative 
      vitreoretinopathy.
PG  - 900-6
AB  - PURPOSE: To measure the levels of interleukins (IL) 1 beta, 6, and 8, and tumor 
      necrosis factor-alpha (TNF alpha) in the vitreous of patients with proliferative 
      vitreoretinopathy (PVR), proliferative diabetic retinopathy (PDR), vitreous 
      hemorrhage, and macular pucker. METHODS: Vitreous samples were collected, 
      undiluted, from patients with PVR, PDR of varying severity, and miscellaneous 
      lesions (vitreous hemorrhage from trauma, macular degeneration, vein occlusion, 
      and non-PVR patients with giant tear, retinal detachment, and macular pucker). 
      Immunoreactive levels of the cytokines, IL-1 beta, IL-6, IL-8, and TNF alpha were 
      determined by enzyme-linked immunoadsorbent assays, and samples were analyzed for 
      protein and hyaluronic acid content using standard assays. RESULTS: The levels of 
      TNF alpha were below detection limits of the assay (< 3 pg/ml). In 45 of the 47 
      samples tested, IL-1 beta levels also were below detection limits of the assay (< 
      3 pg/ml). IL-6 levels ranged from < 30 to 5487 pg/ml, with the highest values 
      observed in the PVR patients. IL-8 levels ranged from < 20 to 1900 pg/ml, and 
      were consistently high in the miscellaneous group. Some of the PVR patients with 
      C2 and C3 level severity also exhibited IL-8 levels exceeding 100 pg/ml. In a 
      second study, IL-6 content of vitreous from miscellaneous and PVR patients was 
      compared. In this study, significantly elevated levels of IL-6 were observed in 
      the PVR patients (91.5 +/- 18 pg/ml) compared to the miscellaneous group (10.3 
      +/- 3.7 pg/ml) CONCLUSIONS: Elevated levels of IL-6 in the vitreous occur in PVR, 
      implicating a role for this cytokine in the pathogenesis of this ocular disorder.
FAU - Kauffmann, D J
AU  - Kauffmann DJ
AD  - Department of Ophthalmology, New York Medical College, Valhalla.
FAU - van Meurs, J C
AU  - van Meurs JC
FAU - Mertens, D A
AU  - Mertens DA
FAU - Peperkamp, E
AU  - Peperkamp E
FAU - Master, C
AU  - Master C
FAU - Gerritsen, M E
AU  - Gerritsen ME
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cytokines/*analysis
MH  - Diabetic Retinopathy/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eye Diseases/immunology
MH  - Female
MH  - Humans
MH  - Interleukin-6/*analysis
MH  - Male
MH  - Middle Aged
MH  - Retinal Diseases/*immunology
MH  - Vitrectomy
MH  - Vitreous Body/*immunology
MH  - Vitreous Hemorrhage/immunology
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1994 Mar;35(3):900-6.