PMID- 8126467
OWN - NLM
STAT- MEDLINE
DCOM- 19940411
LR  - 20081121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 75 ( Pt 3)
DP  - 1994 Mar
TI  - Identification of major differences in the nucleocapsid protein genes of a Quebec 
      strain and European strains of porcine reproductive and respiratory syndrome 
      virus.
PG  - 681-5
AB  - The sequence of the 3'-terminal region of the genome of Quebec reference strain 
      IAF-exp91 of porcine reproductive and respiratory syndrome virus (PRRSV) was 
      investigated by analysis of four cDNA clones. The 3'-terminal 530 nucleotides 
      (nt) encompassed a large open reading frame with a coding capacity of 123 amino 
      acids (M(r) 13,649). The predicted protein was extremely basic and hence was 
      considered to correspond to the nucleocapsid (N) protein gene. When compared to 
      the homologous sequences of two reference Netherlands strains (Lelystad and 
      isolate 10) of PRRSV, the IAF-exp91 N protein was found to be five amino acids 
      shorter and displayed a high degree of divergence. Overall, IAF-exp91 strain 
      showed identities of 63% and 59% with both reference European strains at the 
      nucleotide and amino acid level, respectively. Two amino acid stretches, STAPM 
      and SQGAS, present respectively at the N- and C-terminal regions of the N protein 
      of European strains, were missing in the IAF-exp91 N protein sequence. The 
      3'-terminal non-coding region (151 nt) of the IAF-exp91 strain was 22 nt longer 
      than that of the European strains. The aligned nucleotide sequence of this 
      non-coding region exhibited an overall identity of 59% with that of the European 
      strains. The Quebec reference strain of PRRSV appeared to be related more closely 
      to equine arteritis virus and lactate dehydrogenase-elevating virus than are the 
      two European strains of the virus. Preliminary data obtained by reverse 
      transcription-PCR experiments, using specific or common oligonucleotide primers, 
      suggested that this approach could be useful for distinguishing between PRRSV 
      strains from different geographic origins.
FAU - Mardassi, H
AU  - Mardassi H
AD  - Centre de recherche en virologie, Institut Armand-Frappier, Universite du Quebec, 
      Laval, Canada.
FAU - Mounir, S
AU  - Mounir S
FAU - Dea, S
AU  - Dea S
LA  - eng
SI  - GENBANK/U02095
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Capsid/*genetics
MH  - Europe
MH  - Genetic Variation/*genetics
MH  - Molecular Sequence Data
MH  - Quebec
MH  - RNA Viruses/*genetics
MH  - Sequence Homology
MH  - Swine
MH  - Swine Diseases/*microbiology
MH  - Viral Core Proteins/*genetics
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 10.1099/0022-1317-75-3-681 [doi]
PST - ppublish
SO  - J Gen Virol. 1994 Mar;75 ( Pt 3):681-5. doi: 10.1099/0022-1317-75-3-681.