PMID- 8127150
OWN - NLM
STAT- MEDLINE
DCOM- 19940412
LR  - 20211203
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 8
IP  - 3
DP  - 1994 Mar
TI  - Lack of reciprocal translocation in BCR-ABL positive Ph-negative chronic myeloid 
      leukaemia.
PG  - 454-7
AB  - We used fluorescence in situ hybridization (FISH) to metaphase chromosomes with 
      BCR and ABL cosmid probes in conjunction with the polymerase chain reaction (PCR) 
      to study the mechanism by which the ABL proto-oncogene is inserted into a 
      morphologically normal chromosome 22 in patients with Ph-negative chronic myeloid 
      leukaemia characterized by the BCR-ABL chimeric gene. In control patients with 
      Ph-positive CML the ABL probe localized to 22q- and the 3' BCR probe localized to 
      9q+. In nine Ph-negative CML patients the ABL probe localized to one normal 
      chromosome 9 and to one 'normal' chromosome 22. Both 5' and 3' BCR probes 
      localized exclusively to the chromosomes 22. By PCR all had evidence of BCR-ABL 
      transcripts, but none had evidence of the reciprocal ABL-BCR gene product that is 
      seen in 70% of the Ph-positive CML patients. These data confirm the view that 
      Ph-negative CML results from insertion of ABL-containing DNA sequences into a 
      normal-appearing chromosome 22 without reciprocal translocation of sequences from 
      chromosome 22 to chromosome 9.
FAU - Lazaridou, A
AU  - Lazaridou A
AD  - Department of Haematology, Royal Postgraduate Medical School, London, UK.
FAU - Chase, A
AU  - Chase A
FAU - Melo, J
AU  - Melo J
FAU - Garicochea, B
AU  - Garicochea B
FAU - Diamond, J
AU  - Diamond J
FAU - Goldman, J
AU  - Goldman J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
GS  - ABL
GS  - BCR
MH  - Adult
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 9
MH  - Chronic Disease
MH  - Female
MH  - Fusion Proteins, bcr-abl/analysis
MH  - Genes, abl
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogenes
MH  - Translocation, Genetic/*genetics
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1994 Mar;8(3):454-7.