PMID- 8129622
OWN - NLM
STAT- MEDLINE
DCOM- 19940412
LR  - 20200304
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 134
IP  - 3-4
DP  - 1994
TI  - Low-incidence latent infection with variant B or roseola type human herpesvirus 6 
      in leukocytes of healthy adults.
PG  - 357-68
AB  - Nested primer-based polymerase chain reaction was employed to determine the 
      frequency of latent infection with human herpesvirus 6 (HHV-6) among healthy 
      adults from Bratislava, Slovak Republic. A 592-bp region, upstream from the gene 
      encoding the putative large tegument protein of HHV-6, was amplified from DNA 
      extracted from peripheral blood mononuclear cells (PBMC) of only one of 29 
      seropositive adults, suggesting that as few as 1 in 10(5) PBMC may be infected 
      with the virus. Direct sequencing of the 592-bp fragment indicated that the virus 
      harbored by the seropositive Slovak subject (designated B38) differed by only 3 
      nucleotides from an HHV-6 variant B strain (R-147) isolated from an American 
      infant with a roseola-like illness and by 32 bases from the variant A strain GS 
      isolated from a patient with lymphadenopathy (5.4% sequence divergence). None of 
      these strains had a deoxyadenosine at base position 1251, when compared to the 
      published sequence of strain GS clone pZVH14. Although this discrepancy did not 
      affect the large tegument protein gene, it altered the predicted amino acid 
      sequences of two putative proteins coded by open-reading frames 1 and 2 (ORF 1 
      and ORF 2) located upstream from this gene.
FAU - Rajcani, J
AU  - Rajcani J
AD  - Laboratory of Central Nervous System Studies, National Institute of Neurological 
      Disorders and Stroke, National Institutes of Health, Bethesda, Maryland.
FAU - Yanagihara, R
AU  - Yanagihara R
FAU - Godec, M S
AU  - Godec MS
FAU - Nagle, J W
AU  - Nagle JW
FAU - Kudelova, M
AU  - Kudelova M
FAU - Asher, D M
AU  - Asher DM
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Viral/analysis/genetics
MH  - Exanthema Subitum/microbiology
MH  - Female
MH  - Herpesviridae Infections/*epidemiology/microbiology
MH  - *Herpesvirus 6, Human/genetics
MH  - Humans
MH  - Incidence
MH  - Leukocytes, Mononuclear/*microbiology
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Slovakia/epidemiology
MH  - Viral Proteins/genetics
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF01310573 [doi]
PST - ppublish
SO  - Arch Virol. 1994;134(3-4):357-68. doi: 10.1007/BF01310573.