PMID- 8129848
OWN - NLM
STAT- MEDLINE
DCOM- 19940415
LR  - 20190920
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 30
IP  - 4
DP  - 1993 Dec
TI  - Differences between human sperm and somatic cell DNA in CpG methylation within 
      the HLA class I chromosomal region.
PG  - 228-38
AB  - PROBLEM: We investigated the possible negative regulatory mechanisms that repress 
      classical human leukocyte antigen (HLA) class I gene expression in human 
      spermatozoa and searched for novel testis-specific coding sequences that might be 
      present in MHC class I chromosomal region. METHOD: We performed a comparative DNA 
      methylation analysis of this genomic region in both purified human spermatozoa 
      and mononuclear blood cells from the same donors, using methylation-sensitive 
      restriction enzymes followed by classical or pulsed field gel electrophoresis and 
      hybridization with HLA class I locus-specific probes. RESULTS: Unmethylated CpG 
      sites were detected in the 3' part of HpaII tiny fragments of the HLA-F and HLA-G 
      genes in spermatozoal DNA. In contrast, no difference was observed in the 
      methylation status of the HLA-B, HLA-C, and HLA-E genes between germ and somatic 
      cells. CpG unmethylation events were also detected in several parts of this 
      chromosomal region (outside the known loci) in spermatozoal DNA. CONCLUSIONS: 
      These results suggest that this genomic region undergoes changes in its DNA 
      methylation pattern during the developmental process. We hypothesize that these 
      dynamic changes have functional importance, including a possible transcriptional 
      activity of nonclassical class I genes and/or as yet undescribed testis-specific 
      coding sequences.
FAU - Guillaudeux, T
AU  - Guillaudeux T
AD  - Institut National de la Sante et de la Recherche Medicale, Unite 100, Hopital de 
      Pontchaillou Rennes, France.
FAU - D'Almeida, M
AU  - D'Almeida M
FAU - Girr, M
AU  - Girr M
FAU - Rodriguez, A M
AU  - Rodriguez AM
FAU - Pontarotti, P
AU  - Pontarotti P
FAU - Fauchet, R
AU  - Fauchet R
FAU - Le Bouteiller, P
AU  - Le Bouteiller P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (DNA Probes)
RN  - 0 (HLA Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - HLA-A
GS  - HLA-C
GS  - HLA-E
GS  - HLA-F
GS  - HLA-G
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - DNA/*chemistry/*genetics
MH  - DNA Probes
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - *Genes, MHC Class I
MH  - HLA Antigens/genetics
MH  - Humans
MH  - Leukocytes, Mononuclear/chemistry/immunology
MH  - Male
MH  - Methylation
MH  - Nucleic Acid Hybridization
MH  - Spermatozoa/*chemistry/*immunology
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1111/j.1600-0897.1993.tb00624.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1993 Dec;30(4):228-38. doi: 
      10.1111/j.1600-0897.1993.tb00624.x.