PMID- 8130133
OWN - NLM
STAT- MEDLINE
DCOM- 19940419
LR  - 20041117
IS  - 0921-8912 (Print)
IS  - 0921-8912 (Linking)
VI  - 6
IP  - 1
DP  - 1994 Jan
TI  - Simultaneous characterization of in vitro growing cells by fluorescence in situ 
      hybridization (FISH) and Ki-67 labeling.
PG  - 83-7
AB  - Because cytogenetic analysis and fluorescence in situ hybridization (FISH) 
      techniques fail to provide information about the immunological phenotype with 
      respect to differentiation and malignancy of in vitro growing cells, we developed 
      a system which combines immunophenotyping and FISH. Using immunological detection 
      of the proliferation fraction in short term human cell cultures from different 
      origins by Ki-67 labeling and genetic characterization by FISH, both signals were 
      detectable simultaneously on the same monolayer. Because determination of the 
      proliferation behavior of tumor cells by Ki-67 labeling has now become a 
      widespread laboratory tool, this particular technique may be very useful in tumor 
      cell characterization. This approach allows genetic changes to be assigned to a 
      particular immunological phenotype of individual cells.
FAU - Steilen, H
AU  - Steilen H
AD  - Inst. of Human Genetics, University of the Saar, Homburg/Saar, FRG.
FAU - Burk, U
AU  - Burk U
FAU - Zwergel, T
AU  - Zwergel T
FAU - Wullich, B
AU  - Wullich B
FAU - Unteregger, G
AU  - Unteregger G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Anal Cell Pathol
JT  - Analytical cellular pathology : the journal of the European Society for 
      Analytical Cellular Pathology
JID - 8911016
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - *Cell Division
MH  - Cell Line, Transformed
MH  - Genotype
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Karyotyping
MH  - Ki-67 Antigen
MH  - Male
MH  - Neoplasm Proteins/*analysis
MH  - Nuclear Proteins/*analysis
MH  - Phenotype
MH  - Prostatic Neoplasms/pathology
MH  - Tumor Cells, Cultured
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Anal Cell Pathol. 1994 Jan;6(1):83-7.