PMID- 8130394
OWN - NLM
STAT- MEDLINE
DCOM- 19940415
LR  - 20190512
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 3
IP  - 6
DP  - 1993 Dec
TI  - Lectin binding by macroglial and microglial cells in the brains of young normal 
      and myelin-deficient (md) mutant rats.
PG  - 627-31
AB  - To distinguish macroglia (oligodendrocytes and astrocytes) from microglia in the 
      brain, 'markers' that have been used in previous studies include carbonic 
      anhydrase II (CAII) immunoreactivity for macroglia, and Ricinus and Bandeiraea 
      (BS-I) lectins for microglia. However, in rats < 1 week of age, many cells 
      stained intensely with both anti-CAII and the labelled BS-I lectin. If some of 
      the BS-I+/CAII+ cells were macroglia, and not microglia, BS-I should no longer be 
      regarded as specific for microglia. To confirm or rule out that possibility, 
      lectin histochemistry and double immunofluorescence staining were performed in 
      tissue from the brains of normal young rats and from the microglial cell-enriched 
      brains of myelin-deficient mutant rats. BS-I+/CAII- cells were found and 
      examined. The BS-I+/CAII- cells resembled macrophages and microglia and did, 
      indeed, differ in sizes and shapes from the BS-I+/CA+ cells. The BS-I+/CA+ cells 
      appeared to represent CAII+ putative oligodendrocyte precursors described 
      previously. Although less obvious, a lectin-binding structure was also observed 
      in astrocytes. Lectins may cross-react with macroglial glycoproteins. For 
      example, a glycoprotein found in oligodendrocytes and myelin, the 
      myelin-associated glycoprotein (MAG), is related to the Ig superfamily and cell 
      adhesion molecules. Therefore, it is cautioned that lectins and antibodies 
      against members of the latter families of proteins should be used as cell-type 
      specific markers only if other parameters are also examined.
FAU - Cammer, W
AU  - Cammer W
AD  - Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
FAU - Zhang, H
AU  - Zhang H
LA  - eng
GR  - NS-12890/NS/NINDS NIH HHS/United States
GR  - NS-13792/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Lectins)
RN  - 0 (Myelin Proteins)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Binding Sites
MH  - Brain/cytology/growth & development/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Lectins/*metabolism
MH  - Myelin Proteins/*deficiency
MH  - Neuroglia/cytology/*metabolism
MH  - Rats
MH  - Rats, Mutant Strains
MH  - Rats, Wistar
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1093/glycob/3.6.627 [doi]
PST - ppublish
SO  - Glycobiology. 1993 Dec;3(6):627-31. doi: 10.1093/glycob/3.6.627.