PMID- 8132380
OWN - NLM
STAT- MEDLINE
DCOM- 19940421
LR  - 20190920
IS  - 0105-6263 (Print)
IS  - 0105-6263 (Linking)
VI  - 16
IP  - 6
DP  - 1993 Dec
TI  - Effect of age on seminiferous tubule protein secretion and the adverse effects of 
      testicular toxicants in the rat.
PG  - 370-9
AB  - This study has assessed the effect of age on protein secretion by seminiferous 
      tubules (ST) isolated from rats and their response to Sertoli cell toxicants. ST 
      were isolated from immature (aged 28 days), late pubertal (aged 45 days) and 
      young adult (aged 70 days) rats and cultured in vitro for 24 h with 
      35S-methionine in the presence or absence of FSH (1 mg/ml), m-dinitrobenzene 
      (m-DNB) or nitrobenzene (NB) (both at 10(-4)M). Incorporation of 35S-methionine 
      into newly synthesized proteins in the culture medium (secreted proteins) was 
      assessed and the pattern of protein secretion evaluated using two-dimensional 
      sodium dodecylsulphate polyacrylamide gel electrophoresis (2-D SDS-PAGE). These 
      data were compared with those obtained using cultures of immature rat Sertoli 
      cells+germ cells (SC+GC). Addition of FSH, m-DNB or NB in vitro either had no 
      effect (NB) or had a small stimulatory effect (FSH and m-DNB) on the 
      incorporation of 35S-methionine into overall secreted proteins by ST isolated 
      from immature rats. At the same doses, addition of either FSH, m-DNB or NB to 
      SC+GC co-cultures resulted in increased incorporation of radiolabel into secreted 
      proteins in all instances. In contrast, the same additions to ST isolated from 
      adult rats resulted in a 20-34% decrease in the overall incorporation of 
      35S-methionine into secreted proteins. ST isolated from late pubertal rats showed 
      a similar response to adult rats except that the decreases in incorporation 
      induced by FSH, m-DNB and NB were smaller. Analysis by 2-D SDS-PAGE revealed 
      considerable age-dependent differences in the proteins secreted by ST from 
      immature and adult rats, of which 13 were identified as being of potential 
      importance. Most of these proteins were prominent secretory products of ST from 
      adult rats, but were minor or non-detectable products of cultures of ST or SC+GC 
      from immature rats. Most of these proteins disappeared or decreased in abundance 
      after culture of ST with m-DNB or NB. Two proteins showed the reverse pattern, 
      being more prominent secretory products in immature than mature rats, and their 
      secretion was unaffected or was increased by toxicant exposure. These results 
      demonstrate that there are major age-dependent differences in the secretion of 
      total and specific proteins by isolated ST and that these are probably related to 
      changes in the germ cell complement with age.(ABSTRACT TRUNCATED AT 400 WORDS)
FAU - McLaren, T T
AU  - McLaren TT
AD  - MRC Reproductive Biology Unit, Edinburgh, Scotland, UK.
FAU - Foster, P M
AU  - Foster PM
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Proteins)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - AE28F7PNPL (Methionine)
RN  - DK8B627BU0 (3-dinitrobenzene)
RN  - E57JCN6SSY (nitrobenzene)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Cells, Cultured
MH  - Culture Techniques
MH  - Dinitrobenzenes/pharmacology/toxicity
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Male
MH  - Methionine/pharmacology/toxicity
MH  - Nitrobenzenes/pharmacology/toxicity
MH  - Proteins/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/*metabolism
MH  - Testis/*drug effects
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1111/j.1365-2605.1993.tb01364.x [doi]
PST - ppublish
SO  - Int J Androl. 1993 Dec;16(6):370-9. doi: 10.1111/j.1365-2605.1993.tb01364.x.