PMID- 8136526
OWN - NLM
STAT- MEDLINE
DCOM- 19940425
LR  - 20061115
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 198
IP  - 3
DP  - 1993 Nov
TI  - Expression of tumor necrosis factor-alpha (TNF alpha)-cross-reactive proteins 
      during early chick embryo development.
PG  - 225-39
AB  - We have investigated the expression of tumor necrosis factor-alpha (TNF 
      alpha)-cross-reactive proteins during the early development of the chick embryo 
      from day 1 to day 6 (H-H stages 5-29) using a polyclonal antibody and two 
      monoclonal antibodies to recombinant mouse TNF alpha. We have confirmed the 
      cross-reactivity of the antibodies with chicken tissue in Western blotting 
      studies. Proteins of 50 kDa and 70 kDa, showing anti-TNF alpha cross-reactivity, 
      have been identified during early chick development. In addition, both monoclonal 
      antibodies recognize a 120 kDa protein. These molecules probably represent 
      cytosolic or transmembrane TNF-alpha-like proteins, similar to those previously 
      identified on the surface of cytotoxic T-lymphocytes. We show by ultrastructural 
      cytochemistry that immunoreactivity can be detected at the surfaces of some 
      cells, suggesting that at least some of the antigen is membrane-associated. The 
      proteins are shown to have a widespread tissue distribution during this period of 
      development. Immunoreactivity is first detected in the gastrulating embryo, in 
      the mesoderm and the endoderm. By day 2, expression is confined to the ectoderm 
      and the endoderm, while at day 3 expression appears in the myotome, the 
      notochord, and in nervous tissue. At day 4 the distribution of reactivity is more 
      extensive and includes the notochord, the sclerotome, and the myotome, while the 
      cranial and spinal nerves also become intensely immunoreactive. Also at this 
      stage, neural tube reactivity becomes localized to the marginal neuroepithelial 
      zone, and the lens fibers become positive. This distribution of staining then 
      persists until 6 days of development. We hypothesize that the expression of TNF 
      alpha-cross-reactive proteins in early development could be indicative of a role 
      for them in programmed cell death (apoptosis) during differentiation of the 
      notochord, the lens, and the nervous system, and in tissue remodeling.
FAU - Wride, M A
AU  - Wride MA
AD  - Department of Physiology, University of Alberta, Edmonton, Canada.
FAU - Sanders, E J
AU  - Sanders EJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 0 (Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Blotting, Western
MH  - Chick Embryo/*chemistry/growth & development
MH  - Cross Reactions
MH  - Immunohistochemistry
MH  - Proteins/*analysis
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
AID - 10.1002/aja.1001980308 [doi]
PST - ppublish
SO  - Dev Dyn. 1993 Nov;198(3):225-39. doi: 10.1002/aja.1001980308.