PMID- 8141274
OWN - NLM
STAT- MEDLINE
DCOM- 19940428
LR  - 20180109
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 266
IP  - 2 Pt 1
DP  - 1994 Feb
TI  - Rat skeletal muscle hexokinase II mRNA and activity are increased by a single 
      bout of acute exercise.
PG  - E171-8
AB  - This study addresses the potential role of skeletal muscle hexokinase (HK) II in 
      the regulation of glucose uptake and metabolism in vivo. Male rats undertook a 
      single bout of treadmill exercise and were then killed immediately or after a 
      predetermined recovery period. Three muscles [soleus (Sol), 
      gastrocnemius/plantaris (Gc), and white vastus] were excised, and HK II mRNA, 
      GLUT-4 mRNA, total HK (HK I and HK II) and heat-stable HK (predominantly HK I) 
      activities were assessed. Three hours after the cessation of a single bout of 
      exhaustive exercise, HK II mRNA was significantly increased in all three muscles. 
      Ninety or thirty minutes of exercise, with a 3-h recovery, increased Gc HK II 
      mRNA to the same extent as exhaustive exercise, but 15 min of exercise had no 
      effect. Gc HK II mRNA continued to increase up to 8 h after the cessation of 90 
      min of exercise but returned to basal by 24 h postexercise. In contrast to HK II 
      mRNA, Gc GLUT-4 mRNA was unchanged at 0, 3, 8, and 24 h after the cessation of 90 
      min of exercise. Total HK activity was significantly increased in Sol and Gc, 8 
      and 24 h after the cessation of 90 min of exercise. Heat-stable HK activity was 
      unchanged in all three muscles. The increase in total HK activity, inferred to be 
      an increase of HK II, may be important in the persistence of the postexercise 
      increase in insulin action.
FAU - O'Doherty, R M
AU  - O'Doherty RM
AD  - Department of Molecular Physiology and Biophysics, Vanderbilt University School 
      of Medicine, Nashville, Tennessee 37232.
FAU - Bracy, D P
AU  - Bracy DP
FAU - Osawa, H
AU  - Osawa H
FAU - Wasserman, D H
AU  - Wasserman DH
FAU - Granner, D K
AU  - Granner DK
LA  - eng
GR  - DK-20593/DK/NIDDK NIH HHS/United States
GR  - DK-35107/DK/NIDDK NIH HHS/United States
GR  - DK-42488/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Slc2a4 protein, rat)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Drug Stability
MH  - Glucose Transporter Type 4
MH  - Hexokinase/*genetics/*metabolism
MH  - Hot Temperature
MH  - Male
MH  - Molecular Sequence Data
MH  - Monosaccharide Transport Proteins/genetics
MH  - *Muscle Proteins
MH  - Muscles/*metabolism
MH  - Oligonucleotide Probes/genetics
MH  - Physical Endurance
MH  - *Physical Exertion
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1152/ajpendo.1994.266.2.E171 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Feb;266(2 Pt 1):E171-8. doi: 10.1152/ajpendo.1994.266.2.E171.