PMID- 8142262
OWN - NLM
STAT- MEDLINE
DCOM- 19940504
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 69
IP  - 4
DP  - 1994 Apr
TI  - p53 mutations in cervical carcinogenesis--low frequency and lack of correlation 
      with human papillomavirus status.
PG  - 732-7
AB  - p53 gene aberrations are common in human malignancies, and recent studies suggest 
      that in cervical carcinoma p53 function is inactivated either by complex 
      formation with human papillomavirus (HPV) E6 product or by gene mutation. Using 
      polymerase chain reaction (PCR) followed by denaturing gradient gel 
      electrophoresis (DGGE), we examined the mutational status of the four 'hotspot' 
      regions of the p53 gene in 47 primary cervical carcinomas. HPV status was 
      determined, also by PCR. In 20 of these cases, we examined for loss of 
      heterozygosity (LOH) on chromosome 17p13. In the 47 carcinomas, and in a further 
      68 biopsy specimens from normal, premalignant and malignant cervix, we 
      investigated aberrant immunocytochemical expression of p53. Immunocytochemically, 
      abnormal p53 expression was detected in 13 of 115 cases (8/57 carcinomas). 
      Somatic mutation in p53 was detected in 1 of 47 cervical carcinomas; 36 were 
      positive for HPV 16, 18 or 33. A low level of allele loss (3 out of 20 cases) was 
      detected on chromosome 17p, occurring in both HPV-positive and HPV-negative 
      cases, and in cases with and without p53 mutations. We conclude that somatic 
      mutation in the hotspot regions of the p53 gene occurs infrequently in cervical 
      carcinomas; that immunocytochemically detectable levels of p53 are also 
      infrequent; and that there is no consistent correlation between p53 mutational 
      status, LOH on chromosome 17p or HPV status in these cancers.
FAU - Busby-Earle, R M
AU  - Busby-Earle RM
AD  - Department of Pathology, Edinburgh University Medical School, UK.
FAU - Steel, C M
AU  - Steel CM
FAU - Williams, A R
AU  - Williams AR
FAU - Cohen, B
AU  - Cohen B
FAU - Bird, C C
AU  - Bird CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (DNA Probes, HPV)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adenocarcinoma/genetics
MH  - Carcinoma, Adenosquamous/genetics
MH  - Carcinoma, Squamous Cell/genetics
MH  - Chromosomes, Human, Pair 17
MH  - DNA Mutational Analysis
MH  - DNA Probes, HPV
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Female
MH  - Gene Deletion
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Middle Aged
MH  - *Mutation
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Polymerase Chain Reaction
MH  - Tumor Suppressor Protein p53/analysis
MH  - Uterine Cervical Dysplasia/*genetics/virology
MH  - Uterine Cervical Neoplasms/*genetics/*virology
PMC - PMC1968818
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1038/bjc.1994.138 [doi]
PST - ppublish
SO  - Br J Cancer. 1994 Apr;69(4):732-7. doi: 10.1038/bjc.1994.138.