PMID- 8142658
OWN - NLM
STAT- MEDLINE
DCOM- 19940505
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 83
IP  - 7
DP  - 1994 Apr 1
TI  - Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in 
      chronic myeloid and acute lymphoblastic leukemia by in situ hybridization.
PG  - 1922-8
AB  - The presence of BCR-ABL fusion genes has important diagnostic and prognostic 
      implications in chronic myeloid leukemia (CML) and acute lymphoblastic leukemia 
      (ALL). The CML-specific chimeric BCR-ABL gene with a break involving the major 
      breakpoint cluster region (M-bcr) of the BCR-gene has been detected by means of 
      fluorescence in situ hybridization (FISH). In this study, we present a FISH 
      protocol that allows the detection of breaks in both the major and the minor 
      breakpoint cluster region (m-bcr). Three hybridization signals of D107F9, a yeast 
      artificial chromosome (YAC)-derived probe spanning the breakpoint regions of the 
      BCR gene, were indicative of the translocation events. To increase the 
      specificity further, this probe was combined with cos-abl 8, a cosmid probe 
      flanking the breakpoint within the ABL gene for dual-color hybridization. Samples 
      of 21 patients with CML, the ALL-derived cell line SUP-B15, and of seven patients 
      with Philadelphia chromosome (Ph1)-positive ALL (three of them with breakpoints 
      within m-bcr) were examined. BCR-ABL fusion was detected in all cases with high 
      specificity (false-positive nuclei: mean, 0.1%). On cytogenetic preparations, the 
      percentages of BCR-ABL-positive interphase cells ranged from 53% to 91%. 
      Comparable efficiencies were achieved on blood smears. In conclusion, 
      hybridization with D107F9 and cos-abl 8 allows unambiguous diagnosis of BCR-ABL 
      genes and is likely to become an important tool for the monitoring of therapies 
      in patients with CML and ALL.
FAU - Bentz, M
AU  - Bentz M
AD  - Medizinische Klinik, Universitat Heidelberg, Germany.
FAU - Cabot, G
AU  - Cabot G
FAU - Moos, M
AU  - Moos M
FAU - Speicher, M R
AU  - Speicher MR
FAU - Ganser, A
AU  - Ganser A
FAU - Lichter, P
AU  - Lichter P
FAU - Dohner, H
AU  - Dohner H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Bone Marrow/metabolism
MH  - Cloning, Molecular
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - *Proto-Oncogenes
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - S0006-4971(20)73835-8 [pii]
PST - ppublish
SO  - Blood. 1994 Apr 1;83(7):1922-8.