PMID- 8143093 OWN - NLM STAT- MEDLINE DCOM- 19940505 LR - 20191101 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 1 IP - 1 DP - 1993 May TI - Scanning electron microscopy of synaptonemal complexes. PG - 9-13 AB - The novel application of scanning electron microscopy to study whole-mount surface-spread synaptonemal complex complements of rye (Secale cereale) and rat (Rattus norvegicus) is described. Scanning electron microscopy is able to resolve the third dimension in such preparations and improve the tracing of the continuity of lateral elements without losing information that could be obtained by conventional transmission electron microscopy. This improvement is likely to benefit detailed studies of chromosome synapsis and karyology, and may provide a means of circumventing technical obstacles inhibiting the use of surface-spreads as substrates for in situ hybridization under the electron microscope. FAU - Barlow, A L AU - Barlow AL AD - E. M. Centre, Glasgow University, UK. FAU - Jenkins, G AU - Jenkins G FAU - ap Gwynn, I AU - ap Gwynn I LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 SB - IM MH - Animals MH - Chromosomes/*ultrastructure MH - In Situ Hybridization MH - Male MH - Meiosis MH - Microscopy, Electron MH - Microscopy, Electron, Scanning MH - Rats MH - Secale/cytology/genetics MH - Spermatocytes/ultrastructure MH - *Synaptonemal Complex EDAT- 1993/05/01 00:00 MHDA- 1993/05/01 00:01 CRDT- 1993/05/01 00:00 PHST- 1993/05/01 00:00 [pubmed] PHST- 1993/05/01 00:01 [medline] PHST- 1993/05/01 00:00 [entrez] AID - 10.1007/BF00710602 [doi] PST - ppublish SO - Chromosome Res. 1993 May;1(1):9-13. doi: 10.1007/BF00710602.