PMID- 8144191
OWN - NLM
STAT- MEDLINE
DCOM- 19940502
LR  - 20190825
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 39
IP  - 1
DP  - 1993 Dec
TI  - Skewed distribution of TCR V alpha 7-bearing T cells within tumor-infiltrating 
      lymphocytes of HLA-A24(9)-positive patients with malignant glioma.
PG  - 53-64
AB  - The identification and propagation of T cells with anti-tumor reactivity is 
      critical for understanding the human immune response to tumors, which may 
      possibly be useful in the successful implementation of adoptive immunotherapy 
      against cancer. In order to address this question, we examined the diversity of 
      mRNA transcripts of T-cell receptor (TCR) V alpha and V beta genes in 
      tumor-infiltrating lymphocytes (TIL) of 12 glioma specimens obtained at surgery. 
      Using the polymerase chain reaction (PCR) method and primers for 18 different 
      human TCR V alpha and 22 V beta families to analyze TCR V-(D)-J-C gene 
      rearrangements, we detected a limited expression of TCR variable region, V alpha 
      genes and predominant usage of V alpha 7 within glioma TIL. TCR V beta gene usage 
      was more diverse than that for V alpha, but TCR V beta 13.1 was dominantly 
      expressed in 9 out of 12 patients. In addition, we analyzed the percentage of 
      each V alpha- and V beta-bearing T-cell subpopulation in TIL as well as in 
      peripheral blood lymphocytes (PBL) quantitatively. The distribution of T-cell 
      subpopulation bearing each V alpha or V beta subfamily was variable and uneven in 
      all cases. In 3 cases, the distribution of V alpha 7-bearing T cells in TIL was 
      far higher than in PBL. This phenomenon was not found in T cells bearing TCR V 
      beta 13.1. We also performed human leukocyte antigen (HLA) typing in these 
      patients, and A24(9) was observed in 8 out of 11 patients. Among them all 3 
      patients who showed a skewed distribution of V alpha 7-bearing T cells in TIL 
      expressed HLA-A24(9). There was no correlation between particular class I or II 
      type and TCR V beta gene usage. From these results, it was strongly suggested 
      that T cells bearing TCR V alpha 7 might be targeted to antigenic determinants on 
      glioma cells, and such T-cell population may be useful as effector cells for 
      cancer immunotherapy.
FAU - Ebato, M
AU  - Ebato M
AD  - Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
FAU - Nitta, T
AU  - Nitta T
FAU - Yagita, H
AU  - Yagita H
FAU - Sato, K
AU  - Sato K
FAU - Okumura, K
AU  - Okumura K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (DNA Primers)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Brain Neoplasms/genetics/*immunology
MH  - Child, Preschool
MH  - DNA Primers
MH  - Female
MH  - Gene Expression
MH  - Glioma/genetics/*immunology
MH  - HLA-A Antigens/*immunology
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Lymphocytes, Tumor-Infiltrating/*immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Neoplasm/analysis
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics/*immunology
MH  - T-Lymphocytes/*immunology
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 0165-2478(93)90164-W [pii]
AID - 10.1016/0165-2478(93)90164-w [doi]
PST - ppublish
SO  - Immunol Lett. 1993 Dec;39(1):53-64. doi: 10.1016/0165-2478(93)90164-w.