PMID- 8145010
OWN - NLM
STAT- MEDLINE
DCOM- 19940505
LR  - 20061115
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 123
IP  - 4
DP  - 1994 Apr
TI  - Identification of a murine high-proliferative-potential colony-forming cell 
      (HPP-CFC) capable of producing a number of megakaryocytes and replating for 
      secondary HPP-CFCs in culture.
PG  - 610-6
AB  - The high-proliferative-potential colony-forming cell (HPP-CFC) has been shown to 
      be clearly heterogeneous. Hierarchical subpopulations can be identified by 
      analyzing the kinetics of cell regeneration and the specific cellular requirement 
      for cytokines. In this study, a new type of HPP-CFC, termed high-proliferative 
      potential mixed colony-forming unit-megakaryocyte (HPP-mCFU-MK), was detected in 
      murine bone marrow cell cultures. The HPP-mCFU-MK was able to form macroscopic 
      colonies that fit the criteria of the HPP-CFC colony but contained a number of 
      megakaryocytes. Its growth was stimulated by either aplastic anemia serum (AAS) 
      or a combination of three or more recombinant hematopoietic growth factors but 
      was not inhibited by transforming growth factor-beta 1 (TGF-beta 1) and platelet 
      factor 4 (PF4) in vitro. The recloning of the 12-day-old HPP-mCFU-MK colonies 
      picked up from AAS-stimulated primary cultures caused secondary formation of 
      HPP-CFC colonies. These data suggest that HPP-mCFC-MK is a new subset of stem 
      cell characterized by its ability to produce directly a number of megakaryocytes 
      in response to multifactor stimulation, to generate a secondary set of HPP-CFC in 
      replating culture, and to be unaffected by TGF-beta 1 and PF4 treatment.
FAU - Han, Z C
AU  - Han ZC
AD  - Institut des Vaisseaux et du Sang, Hopital Lariboisiere, Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Hematopoietic Cell Growth Factors)
SB  - IM
MH  - Anemia, Aplastic/blood
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cells, Cultured
MH  - Hematopoietic Cell Growth Factors/physiology
MH  - Hematopoietic Stem Cells/*physiology
MH  - Megakaryocytes/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Swine
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
PST - ppublish
SO  - J Lab Clin Med. 1994 Apr;123(4):610-6.