PMID- 8146323
OWN - NLM
STAT- MEDLINE
DCOM- 19940505
LR  - 20061115
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 138
IP  - 1 Suppl
DP  - 1994 Apr
TI  - Intrinsic radiosensitivity and chromosome aberration analysis using fluorescence 
      in situ hybridization in cells of two human tumor cell lines.
PG  - S40-3
AB  - The survival curves for cells of two human tumor cell lines, HT29 and MeWo, have 
      been defined using a Dynamic Microscopic Imaging Processing Scanner (DMIPS). 
      There are two major differences between these two cell lines: (a) HT29 is more 
      radioresistant than MeWo (surviving fraction at 2 Gy of 74 and 27%, respectively) 
      and (b) HT29 presents a marked multiphasic survival curve with hypersensitivity 
      at low doses (< 0.5 Gy) followed by an increase in radioresistance at higher 
      doses which we have interpreted as "induced radioresistance"; this phenomenon is 
      much less pronounced for the more radiosensitive cell line MeWo. We have now 
      measured in these two cell lines the stable chromosomal aberrations and 
      fragments, with the method of fluorescence in situ hybridization (FISH). We have 
      analyzed chromosome 4, which does not have spontaneous translocations in either 
      of these two cell lines. A dose-effect relationship was studied for radiation 
      doses up to 5 Gy. At all doses, both translocations and breaks are more frequent 
      in the radiosensitive cell line MeWo compared to the radioresistant cell line 
      HT29. The correlation between survival and translocations is different for HT29 
      and MeWo, thus indicating that another factor(s) may be involved in cell killing 
      in these lines.
FAU - Lambin, P
AU  - Lambin P
AD  - Departement de Radiotherapie, Academisch Ziekenhuis St Rafael, Leuven, Belgium.
FAU - Coco-Martin, J
AU  - Coco-Martin J
FAU - Legal, J D
AU  - Legal JD
FAU - Begg, A C
AU  - Begg AC
FAU - Parmentier, C
AU  - Parmentier C
FAU - Joiner, M C
AU  - Joiner MC
FAU - Malaise, E P
AU  - Malaise EP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Cell Survival/radiation effects
MH  - Chromosome Aberrations
MH  - Chromosomes/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Radiation Tolerance
MH  - Tumor Cells, Cultured
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1994 Apr;138(1 Suppl):S40-3.