PMID- 8146594
OWN - NLM
STAT- MEDLINE
DCOM- 19940503
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 39
IP  - 4
DP  - 1994 Apr
TI  - Lipoxin A4 induces neutrophil-dependent cytotoxicity for human endothelial cells.
PG  - 351-4
AB  - The authors have assessed the capacity of neutrophil granulocytes (PMN) to kill 
      cultured human umbilical-vein endothelial cells (HUVEC) in vitro (as release of 
      51Cr) in response to the recently described double dioxygenation product of 
      arachidonic acid, lipoxin A4 (LXA4). LXA4 conferred a marked cytotoxicity, 
      whereas formyl-methionyl-leucyl-phenylalanine (fMLP) was less potent. The LXA4 
      and fMLP effects were dose dependent, with a maximum at 100 nM (which caused 2.7- 
      and 2.3-fold increases of 51Cr release, respectively, relative to buffer-treated 
      controls). The LXA4 and fMLP responses increased with the PMN concentration, 
      depended on the fetal calf serum concentration, incubation temperature and 
      duration and the presence of calcium and magnesium ions.
FAU - Bratt, J
AU  - Bratt J
AD  - Department of Rheumatology, Karolinska Institute, Stockholm, Sweden.
FAU - Lerner, R
AU  - Lerner R
FAU - Ringertz, B
AU  - Ringertz B
FAU - Palmblad, J
AU  - Palmblad J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Culture Media)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (Lipoxins)
RN  - 0 (lipoxin A4)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Cells, Cultured
MH  - Culture Media
MH  - Cytotoxicity, Immunologic/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/immunology
MH  - Humans
MH  - Hydroxyeicosatetraenoic Acids/administration & dosage/*pharmacology
MH  - In Vitro Techniques
MH  - *Lipoxins
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/*immunology
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1111/j.1365-3083.1994.tb03385.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1994 Apr;39(4):351-4. doi: 10.1111/j.1365-3083.1994.tb03385.x.