PMID- 8150972
OWN - NLM
STAT- MEDLINE
DCOM- 19940506
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 32
IP  - 2
DP  - 1994 Feb
TI  - Microplate-based DNA hybridization assays for detection of human retroviral gene 
      sequences.
PG  - 547-50
AB  - Nonisotopic, microwell-based DNA hybridization assays for the specific detection 
      of human immunodeficiency virus type 1 (HIV-1) gag, human T-cell lymphotropic 
      virus type I (HTLV-I) pol, and HTLV-II pol DNA sequences were evaluated. The 
      performances of these detection kits (Gene Detective enzyme oligonucleotide 
      assays; Cellular Products, Inc., Buffalo, N.Y.) were assessed by using clinical 
      samples whose infection status were established by amplification by PCR and then 
      liquid hybridization detection by using virus-specific probes. Peripheral blood 
      mononuclear cell lysates from 59 HIV-1-, 35 HTLV-I-, and 19 HTLV-II-infected 
      individuals and from 15 healthy blood donors were used as substrates for PCR 
      amplification. The results of the study demonstrated a clinical sensitivity of 
      100%. In addition, the enzyme oligonucleotide assays were able to detect 1 to 10 
      proviral copies subsequent to PCR amplification, indicating an analytical 
      sensitivity comparable to that of liquid hybridization.
FAU - Dyster, L M
AU  - Dyster LM
AD  - Cellular Products, Inc., Buffalo, New York 14202.
FAU - Abbott, L
AU  - Abbott L
FAU - Bryz-Gornia, V
AU  - Bryz-Gornia V
FAU - Poiesz, B J
AU  - Poiesz BJ
FAU - Papsidero, L D
AU  - Papsidero LD
LA  - eng
GR  - 5VO1-AI-27658/AI/NIAID NIH HHS/United States
GR  - N01-HB-67021/HB/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
GS  - gag
GS  - pol
MH  - Cell Line
MH  - DNA, Viral/blood/*genetics
MH  - Evaluation Studies as Topic
MH  - *Genes, Viral
MH  - Genes, gag
MH  - Genes, pol
MH  - HIV Infections/diagnosis/microbiology
MH  - HIV-1/genetics/isolation & purification
MH  - HTLV-I Infections/diagnosis/microbiology
MH  - HTLV-II Infections/diagnosis/microbiology
MH  - Human T-lymphotropic virus 1/genetics/isolation & purification
MH  - Human T-lymphotropic virus 2/genetics/isolation & purification
MH  - Humans
MH  - Leukocytes, Mononuclear/microbiology
MH  - *Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
MH  - Proviruses/genetics/isolation & purification
MH  - Retroviridae/*genetics/*isolation & purification
MH  - Sensitivity and Specificity
PMC - PMC263074
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
PMCR- 1994/02/01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
PHST- 1994/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.32.2.547-550.1994 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1994 Feb;32(2):547-50. doi: 10.1128/jcm.32.2.547-550.1994.