PMID- 8151768
OWN - NLM
STAT- MEDLINE
DCOM- 19940512
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 68
IP  - 5
DP  - 1994 May
TI  - Structure and transcription of an immediate-early region in the human herpesvirus 
      6 genome.
PG  - 2978-85
AB  - The unique segment of the human herpesvirus 6 (HHV-6) genome is essentially 
      collinear to the unique long DNA segment of another betaherpesvirus, the human 
      cytomegalovirus (HCMV). However, the HHV-6 genomic section that is analogous in 
      position to the major immediate-early (IE) locus of HCMV does not exhibit 
      recognizable sequence homologies. The respective HHV-6 region of 5.5 kbp is 
      flanked on one side by 25 to 28 incomplete tandem repeats of 105 to 110 bp that 
      contain, with one exception, a single KpnI restriction site (KpnI repeats). About 
      250 reiterations of the sequence motif CACATA are located on the other end. We 
      identified two open reading frames of 375 and 2,595 nucleotides, respectively, on 
      one strand. Strand-specific Northern blot analyses with RNA harvested from HHV-6 
      (strain U1102)-infected HSB-2 cells or cord blood lymphocytes revealed two 
      transcripts of about 3.5 and 4.7 kb in the corresponding orientation. Sequence 
      analyses of the respective cDNA clones and primer extension experiments were used 
      to map the mRNAs. The two transcripts are coterminal and multiply spliced and 
      code for the same putative 104.6-kDa protein, but they are initiated from 
      different promoters. Characterization of smaller cDNA clones and Northern 
      blotting with other strand-specific probes showed that singly spliced mRNAs of 
      1.0 and 1.5 kb are transcribed from the opposite strand; they could code for a 
      17.2-kDa polypeptide. Blocking experiments with cycloheximide led to the 
      conclusion that only the 3.5-kb mRNA is synthesized in the absence of protein 
      biosynthesis upon infection with cell-free virus. This identifies a single IE 
      gene of HHV-6 at the genomic position corresponding to the major IE region of 
      HCMV, although the coding content and transcriptional regulation are quite 
      different for these two herpesvirus IE regions.
FAU - Schiewe, U
AU  - Schiewe U
AD  - Institut fur klinische und molekulare Virologie, Erlangen, Germany.
FAU - Neipel, F
AU  - Neipel F
FAU - Schreiner, D
AU  - Schreiner D
FAU - Fleckenstein, B
AU  - Fleckenstein B
LA  - eng
SI  - GENBANK/X83413
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA, Complementary/genetics
MH  - DNA, Viral/*genetics
MH  - Fetal Blood/cytology
MH  - Gene Expression Regulation, Viral
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Lymphocytes/microbiology
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/*biosynthesis/genetics
MH  - RNA, Viral/*biosynthesis/genetics
MH  - Reading Frames/genetics
MH  - Sequence Analysis, DNA
MH  - *Transcription, Genetic
PMC - PMC236787
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
PMCR- 1994/05/01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
PHST- 1994/05/01 00:00 [pmc-release]
AID - 10.1128/JVI.68.5.2978-2985.1994 [doi]
PST - ppublish
SO  - J Virol. 1994 May;68(5):2978-85. doi: 10.1128/JVI.68.5.2978-2985.1994.