PMID- 8151770
OWN - NLM
STAT- MEDLINE
DCOM- 19940512
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 68
IP  - 5
DP  - 1994 May
TI  - Structure and heterogeneity of the a sequences of human herpesvirus 6 strain 
      variants U1102 and Z29 and identification of human telomeric repeat sequences at 
      the genomic termini.
PG  - 3007-14
AB  - The unit-length genome of human herpesvirus 6 (HHV-6) consists of a single unique 
      component (U) bounded by direct repeats DRL and DRR and forms head-to-tail 
      concatemers during productive infection. cis-elements which mediate cleavage and 
      packaging of progeny virions (a sequences) are found at the termini of all 
      herpesvirus genomes. In HHV-6, DRL and DRR are identical and a sequences may 
      therefore also occur at the U-DR junctions to give the arrangement aDRLa-U-aDRRa. 
      We have sequenced the genomic termini, the U-DRR junction, and the DRR.DRL 
      junction of HHV-6 strain variants U1102 and Z29. A (GGGTTA)n motif identical to 
      the human telomeric repeat sequence (TRS) was found adjacent to, but did not 
      form, the termini of both strain variants. The DRL terminus and U-DRR junction 
      contained sequences closely related to that of the well-conserved herpesvirus 
      packaging signal Cn-Gn-Nn-Gn (pac-1), followed by tandem arrays of TRSs separated 
      by single copies of a hexanucleotide repeat. HHV-6 strain U1102 contained repeat 
      sequences not found in HHV-6 Z29. In contrast, the DRR terminus of both variants 
      contained a simple tandem array of TRSs and a close homolog of a herpesvirus 
      pac-2 signal (GCn-Tn-GCn). The DRR.DRL junction was formed by simple head-to-tail 
      linkage of the termini, yielding an intact cleavage signal, pac-2.x.pac-1, where 
      x is the putative cleavage site. The left end of DR was the site of intrastrain 
      size heterogeneity which mapped to the putative a sequences. These findings 
      suggest that TRSs form part of the a sequence of HHV-6 and that the arrangement 
      of a sequences in the genome can be represented as aDRLa-U-a-DRRa.
FAU - Thomson, B J
AU  - Thomson BJ
AD  - Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom.
FAU - Dewhurst, S
AU  - Dewhurst S
FAU - Gray, D
AU  - Gray D
LA  - eng
SI  - GENBANK/L22335
SI  - GENBANK/L22336
SI  - GENBANK/L22337
SI  - GENBANK/X83413
GR  - AI33020/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA, Viral/*genetics
MH  - *Genetic Variation
MH  - *Genome, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Telomere
PMC - PMC236791
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
PMCR- 1994/05/01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
PHST- 1994/05/01 00:00 [pmc-release]
AID - 10.1128/JVI.68.5.3007-3014.1994 [doi]
PST - ppublish
SO  - J Virol. 1994 May;68(5):3007-14. doi: 10.1128/JVI.68.5.3007-3014.1994.