PMID- 8156688
OWN - NLM
STAT- MEDLINE
DCOM- 19940519
LR  - 20061115
IS  - 0009-921X (Print)
IS  - 0009-921X (Linking)
IP  - 301
DP  - 1994 Apr
TI  - Enhancement of periosteal chondrogenesis in vitro. Dose-response for transforming 
      growth factor-beta 1 (TGF-beta 1).
PG  - 271-80
AB  - Transforming growth factor-beta 1 (TGF-beta 1) has been shown to stimulate 
      chondrogenesis in periosteal explants cultured in agarose suspension. In this 
      study, the dose-response curve for such enhancement was measured. Periosteal 
      explants and fascia lata were harvested from two-month-old rabbits, cultured for 
      six weeks with 0, 0.1, 1, 5, 10, 50, or 100 ng/mL TGF-beta 1 in agarose 
      suspension, then analyzed by histomorphometry and quantitative collagen typing. 
      Cartilage was produced by seven of 11 (64%) of the control periosteal explants 
      cultured in agarose suspension without TGF-beta 1. Transforming growth 
      factor-beta 1 enhanced chondrogenesis in a dose-dependent manner in the range 
      0.1-100 ng/mL. It was most effective at 50 ng/mL. At very high doses (50 and 100 
      ng/mL) of TGF-beta 1, even fascia lata control explants exhibited chondrogenesis. 
      These data indicate that TGF-beta 1 can induce differentiation toward cartilage 
      production as well as enhance it once it has been initiated.
FAU - Miura, Y
AU  - Miura Y
AD  - Cartilage and Connective Tissue Laboratory, Mayo Clinic, Rochester, Minnesota 
      55905.
FAU - Fitzsimmons, J S
AU  - Fitzsimmons JS
FAU - Commisso, C N
AU  - Commisso CN
FAU - Gallay, S H
AU  - Gallay SH
FAU - O'Driscoll, S W
AU  - O'Driscoll SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Orthop Relat Res
JT  - Clinical orthopaedics and related research
JID - 0075674
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Cartilage/*drug effects
MH  - Collagen/isolation & purification
MH  - Culture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fascia Lata/cytology
MH  - Histocytochemistry
MH  - Male
MH  - Periosteum/cytology
MH  - Rabbits
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
PST - ppublish
SO  - Clin Orthop Relat Res. 1994 Apr;(301):271-80.