PMID- 8157505 OWN - NLM STAT- MEDLINE DCOM- 19940519 LR - 20190821 IS - 0378-5955 (Print) IS - 0378-5955 (Linking) VI - 73 IP - 1 DP - 1994 Feb TI - Expression of BDNF and NT-3 mRNA in hair cells of the organ of Corti: quantitative analysis in developing rats. PG - 46-56 AB - Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are synthesized by inner and outer hair cells of the developing organ of Corti. This raises the possibility that the reorganization of cochlear innervation patterns that occurs postnatally may be influenced by changing levels of neurotrophin expression. To determine if differential expression of BDNF or NT-3 in the inner and outer hair cells correlates with the reorganization of afferent and efferent innervation, we used in situ hybridization techniques to quantify relative levels of transcript biosynthesis in hair cells of developing rats. BDNF transcripts decreased in inner and outer hair cells from E17 to insignificant levels at P4. NT-3 expression was high at E17 in inner and outer hair cells, decreased in outer hair cells by E21, in inner hair cells by P1, remained low during the first postnatal week and was increased in the adult. The decreases in expression of both neurotrophins at birth precede the retraction of afferent nerve terminals from outer hair cells. BDNF and NT-3 transcription decreases substantially in outer hair cells between E21 and P4 when efferent innervation begins, indicating target biosynthesis of these neurotrophins is not likely to be instrumental in efferent target selection. FAU - Wheeler, E F AU - Wheeler EF AD - Department of Physiology and Biophysics SJ-40, University of Washington School of Medicine, Seattle 98195. FAU - Bothwell, M AU - Bothwell M FAU - Schecterson, L C AU - Schecterson LC FAU - von Bartheld, C S AU - von Bartheld CS LA - eng GR - HD 29177/HD/NICHD NIH HHS/United States GR - HL 43397/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - Netherlands TA - Hear Res JT - Hearing research JID - 7900445 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Neurotrophin 3) RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor MH - Hair Cells, Auditory, Inner/*metabolism MH - Hair Cells, Auditory, Outer/*metabolism MH - Immunohistochemistry MH - In Situ Hybridization MH - Nerve Growth Factors/*biosynthesis MH - Nerve Tissue Proteins/*biosynthesis/genetics MH - Neurotrophin 3 MH - Organ of Corti/embryology/*metabolism MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Transcription, Genetic EDAT- 1994/02/01 00:00 MHDA- 1994/02/01 00:01 CRDT- 1994/02/01 00:00 PHST- 1994/02/01 00:00 [pubmed] PHST- 1994/02/01 00:01 [medline] PHST- 1994/02/01 00:00 [entrez] AID - 0378-5955(94)90281-X [pii] AID - 10.1016/0378-5955(94)90281-x [doi] PST - ppublish SO - Hear Res. 1994 Feb;73(1):46-56. doi: 10.1016/0378-5955(94)90281-x.