PMID- 8158256 OWN - NLM STAT- MEDLINE DCOM- 19940517 LR - 20220310 IS - 0270-6474 (Print) IS - 1529-2401 (Electronic) IS - 0270-6474 (Linking) VI - 14 IP - 4 DP - 1994 Apr TI - Regulation of neurotrophin receptor expression during embryonic and postnatal development. PG - 2054-68 AB - Members of the NGF family of proteins act as neurotrophic agents for defined populations of peripheral and central neurons during embryonic and postnatal development. We have studied the presence of receptors for brain-derived neurotrophic factor (BDNF) and neurotrophin-3 and -4/5 (NT-3, NT-4/5) by cross-linking radioiodinated neurotrophins to specific cell surface receptors. We have identified neurotrophin receptors representing full-length TrkB and TrkC and their truncated forms (lacking a functional cytoplasmic kinase domain) in neuronal as well as in non-neuronal tissues. During chicken embryonic and early postnatal brain development, expression of full-length TrkB and TrkC proteins preceded the onset of the truncated forms of these receptors. A similar pattern was also observed in mouse embryonic and early postnatal brain. The relative levels of neurotrophin receptors in the basal forebrain and in the hippocampus did not change significantly with age in mice. High levels of receptors for the three neurotrophins were detected in the nigrostriatal system. Full-length TrkB and TrkC receptors were found in chicken and rat embryonic ventral spinal cord, as well as on purified motoneurons. Again, truncated TrkB appeared significantly later than the full-length form on spinal motoneurons. In chicken embryonic retina and optic tectum we detected full-length TrkB and TrkC; however, the optic tectum also expressed large amounts of the truncated form of TrkB. TrkC but not TrkB was detected in chicken embryonic skeletal muscle, suggesting that NT-3 may have a novel function in this tissue. The presence of neurotrophin receptors in a wide variety of embryonic and postnatal tissues underlines the significant role of BDNF, NT-3, and NT-4/5 in embryonic and postnatal development. The regulation of the ratio of full-length versus truncated neurotrophin receptors may play an important role in the development, maturation, and maintenance of various neuronal networks. FAU - Escandon, E AU - Escandon E AD - Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080-4990. FAU - Soppet, D AU - Soppet D FAU - Rosenthal, A AU - Rosenthal A FAU - Mendoza-Ramirez, J L AU - Mendoza-Ramirez JL FAU - Szonyi, E AU - Szonyi E FAU - Burton, L E AU - Burton LE FAU - Henderson, C E AU - Henderson CE FAU - Parada, L F AU - Parada LF FAU - Nikolics, K AU - Nikolics K LA - eng GR - N01-CO-74101/CO/NCI NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Immune Sera) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Neurotrophin 3) RN - 0 (Peptides) RN - 0 (Receptor, Ciliary Neurotrophic Factor) RN - 0 (Receptors, Growth Factor) RN - 0 (Recombinant Proteins) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) RN - EC 2.7.10.1 (Receptor, trkC) RN - P658DCA9XD (neurotrophin 4) SB - IM MH - Aging/*metabolism MH - Amino Acid Sequence MH - Animals MH - Binding, Competitive MH - Brain/embryology/growth & development/*metabolism MH - Brain-Derived Neurotrophic Factor MH - Cell Membrane/metabolism MH - Embryo, Mammalian MH - Embryonic and Fetal Development MH - Gene Expression Regulation MH - Gestational Age MH - Immune Sera MH - Kinetics MH - Mice MH - Mice, Inbred BALB C MH - Molecular Sequence Data MH - Motor Neurons/metabolism MH - Nerve Growth Factors/*metabolism MH - Nerve Tissue Proteins/metabolism MH - Neurons/*metabolism MH - Neurotrophin 3 MH - Organ Specificity MH - Peptides/chemical synthesis/immunology MH - Receptor Protein-Tyrosine Kinases/*biosynthesis/isolation & purification/metabolism MH - Receptor, Ciliary Neurotrophic Factor MH - Receptor, trkC MH - Receptors, Growth Factor/*biosynthesis/isolation & purification/metabolism MH - Recombinant Proteins/metabolism PMC - PMC6577118 EDAT- 1994/04/01 00:00 MHDA- 1994/04/01 00:01 PMCR- 1994/10/01 CRDT- 1994/04/01 00:00 PHST- 1994/04/01 00:00 [pubmed] PHST- 1994/04/01 00:01 [medline] PHST- 1994/04/01 00:00 [entrez] PHST- 1994/10/01 00:00 [pmc-release] AID - 10.1523/JNEUROSCI.14-04-02054.1994 [doi] PST - ppublish SO - J Neurosci. 1994 Apr;14(4):2054-68. doi: 10.1523/JNEUROSCI.14-04-02054.1994.