PMID- 8159094
OWN - NLM
STAT- MEDLINE
DCOM- 19940513
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 43
IP  - 4
DP  - 1994 Apr
TI  - Uremia-induced disturbances in hepatic carbohydrate metabolism: enhancement by 
      sucrose feeding.
PG  - 403-12
AB  - A high-sucrose (S) diet accentuates anorexia and stunts growth in uremic (U) 
      rats, and an oral S load induces a greater hyperfructosemia in U rats than in 
      control (C) rats. Four studies were performed to determine the roles of S feeding 
      and an acute S load on liver carbohydrate (CHO) metabolism in U and C rats (eight 
      to 10 rats per group). We also examined the plasma responses to either water or a 
      S load. Levels of the main metabolites of glycolysis, gluconeogenesis, and 
      glycogenesis were measured under basal conditions (7 hours' postmeal) in U and C 
      rats fed either a cornstarch diet (study I) or S diet (study II) and at 30 and 60 
      minutes after an intragastric S load (studies III and IV) in s-fed U and C rats. 
      The weight gain, food intake, and plasma creatinine and urea levels of the rats 
      in the four studies were comparable. Weight gain and liver weight (g/100 g body 
      weight) were lower in U than in C rats. In the plasma, baseline levels of lactate 
      were decreased by uremia and S feeding and those of glucose (G) were increased by 
      S feeding. The increases in plasma G and fructose (F) levels after a S load were 
      greater in U rats than in C rats, whereas those of plasma lactate were 
      comparable. In the liver under basal conditions, uremia markedly decreased levels 
      of glycogen, F-1,6-diphosphate (F-1,6-diP), F-2,6-diP, 3-glycero-phosphate 
      (3-glycero-P), dihydroxyacetone phosphate (DHAP), pyruvate, lactate, and 
      adenosine triphosphate (ATP), and the phosphorylation state (ATP/adenosine 
      diphosphate [ADP] x inorganic phosphorus [PI]), increased phosphoenolpyruvate 
      (PEP), ADP, and Pi levels, but did not affect the cytosolic redox state 
      (pyruvate/lactate). In addition to uremia, S feeding further decreased levels of 
      glycogen, F-2,6-diP, 3-glycero-P, and ATP. After S loading, liver F levels 
      increased more in U than in C rats, but glycogen and 3-glycero-P levels increased 
      less in U than in C rats. Liver lactate and pyruvate levels increased more in U 
      than in C rats, and the pyruvate/lactate and DHAP/3-glycero-P ratios were higher 
      in U than in C rats after a S load. The ATP level and the phosphorylation state 
      in U rats increased 30 minutes later in U than in C rats. Our findings indicate 
      that uremia causes a depletion in liver glycogen, which is enhanced by S feeding 
      and could be partially attributed to decreased glycogen synthesis.(ABSTRACT 
      TRUNCATED AT 400 WORDS)
FAU - Laouari, D
AU  - Laouari D
AD  - INSERM U.192, Hopital des Enfants Malades, Paris, France.
FAU - Jurkovitz, C
AU  - Jurkovitz C
FAU - Burtin, M
AU  - Burtin M
FAU - Bois, B
AU  - Bois B
FAU - Vassault, A
AU  - Vassault A
FAU - Kleinknecht, C
AU  - Kleinknecht C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 57-50-1 (Sucrose)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - *Carbohydrate Metabolism
MH  - Glucose/*metabolism
MH  - Kidney/metabolism
MH  - Liver/*metabolism
MH  - Nutritional Status
MH  - Rats
MH  - Sucrose/administration & dosage/*pharmacology
MH  - Uremia/blood/*metabolism
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 0026-0495(94)90067-1 [pii]
AID - 10.1016/0026-0495(94)90067-1 [doi]
PST - ppublish
SO  - Metabolism. 1994 Apr;43(4):403-12. doi: 10.1016/0026-0495(94)90067-1.