PMID- 8160770
OWN - NLM
STAT- MEDLINE
DCOM- 19940516
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 144
IP  - 4
DP  - 1994 Apr
TI  - Cytomegalovirus inhibits major histocompatibility class II expression on infected 
      endothelial cells.
PG  - 683-92
AB  - Persistent human cytomegalovirus (HCMV) infections are responsible for 
      significant morbidity and mortality in immunocompromised individuals. One 
      mechanism by which HCMV may develop persistence after primary infection is 
      through inhibition of host cell human leukocyte antigen (HLA) class II expression 
      with resultant escape from normal antiviral immune surveillance. 
      Immunofluorescence flow cytometry of human endothelial cell (EC) cultures 
      infected with HCMV AD169 and an EC propagated strain, VHL/E, showed a marked 
      reduction in interferon-gamma (IFN-gamma)-induced surface expression of HLA-DR. 
      This inhibition did not occur when EC were treated with ultraviolet-inactivated 
      virus and IFN-gamma. HCMV, as determined by dual-labeling immunohistochemistry, 
      inhibited induction of surface and cytoplasmic class II antigens specifically in 
      infected cells. HCMV infection also inhibited IFN-gamma and tumor necrosis 
      factor-alpha up-regulation of HLA class I expression. Northern blot analysis of 
      infected, IFN-gamma-treated human umbilical vein endothelial cells revealed an 
      absence of class II mRNA. Persistence of HCMV may result in part from its ability 
      to inhibit HLA class II induction in infected cells.
FAU - Sedmak, D D
AU  - Sedmak DD
AD  - Department of Pathology, Ohio State University College of Medicine, Columbus.
FAU - Guglielmo, A M
AU  - Guglielmo AM
FAU - Knight, D A
AU  - Knight DA
FAU - Birmingham, D J
AU  - Birmingham DJ
FAU - Huang, E H
AU  - Huang EH
FAU - Waldman, W J
AU  - Waldman WJ
LA  - eng
GR  - AI29002/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
CIN - Am J Pathol. 1994 Apr;144(4):637-50. PMID: 8160765
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Cytomegalovirus/*physiology/radiation effects
MH  - Endothelium, Vascular/*microbiology
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Histocompatibility Antigens Class II/genetics/*metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interferon-gamma/metabolism
MH  - *Major Histocompatibility Complex
MH  - RNA, Messenger/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Umbilical Veins
PMC - PMC1887242
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
PMCR- 1994/10/01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1994 Apr;144(4):683-92.