PMID- 8160772
OWN - NLM
STAT- MEDLINE
DCOM- 19940516
LR  - 20220331
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 144
IP  - 4
DP  - 1994 Apr
TI  - Production of monocyte chemoattractant protein-1 and macrophage inflammatory 
      protein-1 alpha by inflammatory granuloma fibroblasts.
PG  - 711-8
AB  - The formation of hepatic granulomas around persistently deposited Schistosoma 
      mansoni eggs leads to parenchymal damage, ongoing fibrosis, and ultimate loss of 
      liver function. In this study, the production of macrophage inflammatory 
      protein-1 alpha (MIP-1) and monocyte chemoattractant protein-1 (MCP-1) by 
      granuloma fibroblasts was examined to establish the potential contribution of 
      intragranuloma fibroblasts to the maintenance of the chronic inflammation. 
      Isolated fibroblasts from dispersed acute infection hepatic granulomas were grown 
      in tissue culture for 3 to 4 weeks and used on the third or fourth passage. We 
      initially surveyed fibroblasts for production of MIP-1 and MCP-1 by reverse 
      transcription-polymerase chain reaction (RT-PCR) after stimulation with 
      interleukin (IL)-1, tumor necrosis factor, interferon (IFN)-gamma, IL-4, or 
      IL-10: cytokines found within the granuloma. These studies demonstrated 
      constitutive expression of MCP-1 and differential up-regulation of MIP-1 on 
      cytokine stimulation. Protein expression was then verified by immunohistochemical 
      localization of MIP-1 and MCP-1 in paraformaldehyde-fixed fibroblasts and by 
      direct quantitation of MIP-1 and MCP-1 in culture supernatants by specific 
      ELISAs. These studies demonstrated constitutive expression of MCP-1 in 
      unstimulated and cytokine-stimulated granuloma fibroblasts. In contrast, IL-1 
      (0.1 to 2.5 ng/ml), IFN-gamma (10 micrograms/ml), and IL-10 (2.5 to 10 ng/ml) 
      were able to induce the significant production of MIP-1 by the granuloma 
      fibroblasts. Interestingly, normal noninflammatory fibroblasts from uninfected 
      mice showed no significant production of MIP-1 or MCP-1 in response to these 
      cytokines. These results suggest that granuloma fibroblasts may be phenotypically 
      altered compared with normal fibroblasts and have a significant role in leukocyte 
      recruitment, granuloma growth, and maintenance of the egg-induced lesion.
FAU - Lukacs, N W
AU  - Lukacs NW
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor.
FAU - Chensue, S W
AU  - Chensue SW
FAU - Smith, R E
AU  - Smith RE
FAU - Strieter, R M
AU  - Strieter RM
FAU - Warmington, K
AU  - Warmington K
FAU - Wilke, C
AU  - Wilke C
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - HL-02401/HL/NHLBI NIH HHS/United States
GR  - HL-35276/HL/NHLBI NIH HHS/United States
GR  - HL-3693/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemokine CCL4
MH  - Chemotactic Factors/*biosynthesis/genetics
MH  - Cytokines/*biosynthesis/genetics
MH  - DNA Primers
MH  - Female
MH  - Fibroblasts/*immunology
MH  - Gene Expression
MH  - Granuloma/*immunology/pathology
MH  - Immunoenzyme Techniques
MH  - Liver Diseases, Parasitic/immunology/pathology
MH  - Macrophage Inflammatory Proteins
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Molecular Sequence Data
MH  - Monokines/*biosynthesis/genetics
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Schistosomiasis mansoni/immunology/pathology
PMC - PMC1887251
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
PMCR- 1994/10/01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1994 Apr;144(4):711-8.