PMID- 8161544
OWN - NLM
STAT- MEDLINE
DCOM- 19940525
LR  - 20220309
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 33
IP  - 16
DP  - 1994 Apr 26
TI  - Solution structure of the amino-terminal fragment of urokinase-type plasminogen 
      activator.
PG  - 4847-64
AB  - The amino-terminal fragment (ATF) of urokinase-type plasminogen activator is a 
      two domain protein which consists of a growth factor and a kringle domain. The 
      1H, 13C, and 15N chemical shifts of this protein have been assigned using 
      heteronuclear two- and three-dimensional NMR experiments on selective and 
      uniformly 15N- and 15N/13C-labeled protein isolated from mammalian cells that 
      overexpress the protein. The chemical shift assignments were used to interpret 
      the NOE data which resulted in a total of 1299 NOE restraints. The NOE restraints 
      were used along with 27 phi angle restraints and 21 hydrogen-bonding restraints 
      to produce 15 low energy structures. The individual domains in the structures are 
      highly converged, but the two domains are structurally independent. The root mean 
      square deviations (rmsd) between residues 11-46 in the growth factor domain and 
      the mean atomic coordinates were 0.99 +/- 0.2 for backbone heavy atoms and 1.65 
      +/- 0.2 for all non-hydrogen atoms. For residues 55-130 in the kringle domain, 
      the rmsd was 0.84 +/- 0.2 for backbone heavy atoms and 1.42 +/- 0.2 for all 
      non-hydrogen atoms. The overall structures of the individual domains are very 
      similar to the structures of homologous proteins. However, important structural 
      differences between the growth factor and other homologous proteins were observed 
      in the region which has been implicated in binding the urokinase receptor which 
      may explain, in part, why other growth factors show no appreciable affinity for 
      the urokinase receptor.
FAU - Hansen, A P
AU  - Hansen AP
AD  - Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, Illinois 
      60064.
FAU - Petros, A M
AU  - Petros AM
FAU - Meadows, R P
AU  - Meadows RP
FAU - Nettesheim, D G
AU  - Nettesheim DG
FAU - Mazar, A P
AU  - Mazar AP
FAU - Olejniczak, E T
AU  - Olejniczak ET
FAU - Xu, R X
AU  - Xu RX
FAU - Pederson, T M
AU  - Pederson TM
FAU - Henkin, J
AU  - Henkin J
FAU - Fesik, S W
AU  - Fesik SW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Solutions)
RN  - 0 (urokinase-type plasminogen activator (6-135))
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Kringles
MH  - Magnetic Resonance Spectroscopy
MH  - Mammals
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Peptide Fragments/*chemistry
MH  - Protein Structure, Secondary
MH  - Recombinant Proteins/chemistry
MH  - Solutions
MH  - Urokinase-Type Plasminogen Activator/*chemistry
EDAT- 1994/04/26 00:00
MHDA- 1994/04/26 00:01
CRDT- 1994/04/26 00:00
PHST- 1994/04/26 00:00 [pubmed]
PHST- 1994/04/26 00:01 [medline]
PHST- 1994/04/26 00:00 [entrez]
AID - 10.1021/bi00182a013 [doi]
PST - ppublish
SO  - Biochemistry. 1994 Apr 26;33(16):4847-64. doi: 10.1021/bi00182a013.