PMID- 8161659
OWN - NLM
STAT- MEDLINE
DCOM- 19940523
LR  - 20190815
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 15
IP  - 1
DP  - 1994 Jan
TI  - Wetting of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) foams for 
      tissue culture.
PG  - 55-8
AB  - Biodegradable foams of hydrophobic polymers can be efficiently wet by two-step 
      immersion in ethanol and water, which overcomes the hindered entry of water into 
      air-filled pores. Ethanol readily enters into the porous polymer, after which it 
      is diluted and replaced by water. This method was evaluated for porous disks of 
      poly(L-lactic acid) (PLLA) and poly(DL-lactic-co-glycolic acid) (PLGA) foams of 
      copolymer ratios 85:15 and 50:50. For PLLA disks of 0.88 porosity and 1730 
      microns thickness, prewetting with ethanol for 1 h increased the percentage of 
      void volume filled with water after 48 h from 23 to 79%. The same enhanced entry 
      of water was also observed for prewet PLGA 85:15 disks of 0.86 porosity and 1300 
      microns thickness, which exhibited an increase from 59 to 97% void volume 
      occupied by water. Furthermore, the water entry even after 1 h was very close to 
      its plateau value for all prewet polymers tested. In recent studies, this method 
      has been useful in uniformly seeding three-dimensional biodegradable polymer 
      substrates for cell and tissue culture.
FAU - Mikos, A G
AU  - Mikos AG
AD  - Department of Chemical Engineering, Massachusetts Institute of Technology, 
      Cambridge 02139.
FAU - Lyman, M D
AU  - Lyman MD
FAU - Freed, L E
AU  - Freed LE
FAU - Langer, R
AU  - Langer R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Lactates)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
SB  - IM
MH  - Culture Techniques
MH  - *Lactates/chemistry
MH  - *Lactic Acid
MH  - Polyesters
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers/chemistry
MH  - Wettability
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 0142-9612(94)90197-X [pii]
AID - 10.1016/0142-9612(94)90197-x [doi]
PST - ppublish
SO  - Biomaterials. 1994 Jan;15(1):55-8. doi: 10.1016/0142-9612(94)90197-x.