PMID- 8161683
OWN - NLM
STAT- MEDLINE
DCOM- 19940520
LR  - 20190606
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 66
IP  - 2 Pt 1
DP  - 1994 Feb
TI  - Analysis of protein binding to receptor-doped lipid monolayers by Monte Carlo 
      simulation.
PG  - 305-9
AB  - This paper presents a Monte Carlo simulation (MCS) method for estimating the 
      parameters that characterize ligand-receptor binding directly from experimentally 
      derived binding isotherms. Binding parameters are estimated by incorporating an 
      MCS algorithm for ligand binding to a two-dimensional receptor array into a 
      nonlinear regression program. The MCS method was tested by analyzing experimental 
      isotherms of avidin binding to biotinylated lipid in Langmuir-Blodgett (LB) 
      monolayers. The MCS-derived cooperativity coefficients and intrinsic association 
      constants for avidin-biotin binding to LB films are correlated strongly (R2 > 
      0.93) with the binding parameters determined from the same experimental data by a 
      thermodynamic equilibrium binding model (Zhao et al. 1993. Langmuir. 
      9:3166-3173). This result shows MCS to be an accurate and potentially more 
      versatile method for characterizing biomolecular interactions at surfaces.
FAU - Zhao, S
AU  - Zhao S
AD  - Department of Biomedical Engineering, Duke University, Durham, North Carolina 
      27708.
FAU - Reichert, W M
AU  - Reichert WM
LA  - eng
GR  - HL 32132/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Lipids)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (fluorescein isothiocyante avidin)
RN  - 1405-69-2 (Avidin)
RN  - 3026-45-7 (1,2-dipalmitoyl-3-phosphatidylethanolamine)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Algorithms
MH  - Avidin/analogs & derivatives/chemistry/metabolism
MH  - Biophysical Phenomena
MH  - Biophysics
MH  - Fluorescein-5-isothiocyanate/analogs & derivatives/chemistry/metabolism
MH  - In Vitro Techniques
MH  - *Lipid Metabolism
MH  - Lipids/chemistry
MH  - Models, Biological
MH  - Monte Carlo Method
MH  - Phosphatidylethanolamines/chemistry/metabolism
MH  - Protein Binding
MH  - Receptors, Cell Surface/chemistry/*metabolism
MH  - Thermodynamics
PMC - PMC1275696
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
PMCR- 1995/02/01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
PHST- 1995/02/01 00:00 [pmc-release]
AID - S0006-3495(94)80778-7 [pii]
AID - 10.1016/s0006-3495(94)80778-7 [doi]
PST - ppublish
SO  - Biophys J. 1994 Feb;66(2 Pt 1):305-9. doi: 10.1016/s0006-3495(94)80778-7.