PMID- 8162702
OWN - NLM
STAT- MEDLINE
DCOM- 19940523
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 66
IP  - 4
DP  - 1994
TI  - Chromosomal mapping of mouse 5S rRNA genes by direct R-banding fluorescence in 
      situ hybridization.
PG  - 246-9
AB  - The mouse 5S rRNA gene was mapped by direct R-banding fluorescence in situ 
      hybridization (FISH) with biotinylated probes. Two genomic fragments amplified by 
      PCR from total genomic DNA of BALB/c mice and Mus spretus, a 0.16-kb fragment 
      that included the 121-bp 5S rRNA gene and a 1.6-kb fragment that included the 
      whole spacer region, were used for chromosomal mapping of the 5S rRNA gene. Both 
      fragments hybridized to a single locus on a pair of autosomal chromosomes of 
      BALB/c mice. The major cluster of mouse 5S rRNA genes was assigned to the most 
      terminal R-negative to R-positive bands of the E region of mouse Chromosome 8, 
      which is homologous to the linkage of the 5S rRNA gene on the long arm of human 
      chromosome 1. The location of the 5S rRNA gene was mapped in five laboratory 
      strains, in wild mice of six Mus musculus subspecies (domesticus, brevirostris, 
      musculus, bactrianus, castaneus, and molossinus) derived from 10 separate 
      localities, and in four different Mus species (spretus, hortulanus, spicilegus, 
      and caroli), using FISH. The 5S rRNA cluster mapped to the same position on the 
      chromosomes of all mouse species and subspecies studied. These results suggest 
      that the location of the mouse 5S rRNA gene on the distal telomeric region of 
      Chromosome 8 is evolutionarily conserved. In comparison, the chromosomal 
      assignments of centromeric 18S-28S rRNA genes are highly variable among the 
      different M. musculus subspecies and Mus species.
FAU - Matsuda, Y
AU  - Matsuda Y
AD  - Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
FAU - Moriwaki, K
AU  - Moriwaki K
FAU - Chapman, V M
AU  - Chapman VM
FAU - Hoi-Sen, Y
AU  - Hoi-Sen Y
FAU - Akbarzadeh, J
AU  - Akbarzadeh J
FAU - Suzuki, H
AU  - Suzuki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - DNA Primers
MH  - DNA Probes
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Mice/*genetics
MH  - Mice, Inbred BALB C/genetics
MH  - Mice, Inbred C3H/genetics
MH  - Mice, Inbred C57BL/genetics
MH  - Mice, Inbred DBA/genetics
MH  - Mice, Inbred ICR/genetics
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Muridae/*genetics
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Restriction Mapping
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1159/000133704 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1994;66(4):246-9. doi: 10.1159/000133704.