PMID- 8163489
OWN - NLM
STAT- MEDLINE
DCOM- 19940526
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 269
IP  - 16
DP  - 1994 Apr 22
TI  - Methylation sensitivity of the enhancer from the human papillomavirus type 16.
PG  - 11902-11
AB  - The human papillomavirus type 16 is associated with anogenital cancer. 
      Transcription of the viral transforming genes E6 and E7 is under the control of 
      an epithelial cell type-specific enhancer. In the enhancer core, we have 
      identified a regulatory element that is recognized by a novel nuclear factor 
      named MSPF (methylation-sensitive papillomavirus transcription factor). Mutating 
      the MSPF binding site strongly affects the enhancer activity. The MSPF 
      recognition sequence 5'-ATGCGNNNNCGCCT-3' contains two CpG dinucleotides, 
      potential targets for 5-cytidine methylation. DNA recognition by MSPF is strictly 
      methylation-sensitive, since introduction of 5-methylcytidine into either CpG 
      abolishes complex formation. Moreover, CpG methylation of the MSPF binding site 
      suppresses the activity of the enhancer and of the MSPF enhanson subfragment in 
      vivo. In the cervical carcinoma cell line CaSki, which has integrated multiple 
      transcriptionally inactive human papilloma virus 16 genomes, a few of the viral 
      genomes are methylated at the MSPF binding site. These findings suggest that 
      viral transcription can be suppressed by methylation of the regulatory region, an 
      event that prevents binding of the cellular transcription factor MSPF.
FAU - List, H J
AU  - List HJ
AD  - Institut fur Biochemie, Technische Hochschule Darmstadt, Germany.
FAU - Patzel, V
AU  - Patzel V
FAU - Zeidler, U
AU  - Zeidler U
FAU - Schopen, A
AU  - Schopen A
FAU - Ruhl, G
AU  - Ruhl G
FAU - Stollwerk, J
AU  - Stollwerk J
FAU - Klock, G
AU  - Klock G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cell Transformation, Neoplastic
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis/metabolism
MH  - DNA, Viral/genetics/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - *Enhancer Elements, Genetic
MH  - *Genes, Viral
MH  - HeLa Cells
MH  - Humans
MH  - Methylation
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Oligodeoxyribonucleotides/chemical synthesis/metabolism
MH  - Papillomaviridae/*genetics/metabolism
MH  - Regulatory Sequences, Nucleic Acid
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1994/04/22 00:00
MHDA- 1994/04/22 00:01
CRDT- 1994/04/22 00:00
PHST- 1994/04/22 00:00 [pubmed]
PHST- 1994/04/22 00:01 [medline]
PHST- 1994/04/22 00:00 [entrez]
AID - S0021-9258(17)32658-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1994 Apr 22;269(16):11902-11.