PMID- 8163522
OWN - NLM
STAT- MEDLINE
DCOM- 19940526
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 269
IP  - 16
DP  - 1994 Apr 22
TI  - Increased amyloid production from aberrant beta-amyloid precursor proteins.
PG  - 12179-84
AB  - The 4-kDa beta-amyloid protein that forms fibrillar deposits in Alzheimer's 
      diseased brains is derived from a large precursor, the beta-amyloid precursor 
      protein (beta-APP). Recently, it has been reported that beta-amyloid is normally 
      produced and secreted by cultured mammalian cells. In our studies involving 
      recombinant expression of beta-APP, increased yields of beta-amyloid were 
      associated with expression of aberrant beta-APP molecules. Deletion mutations 
      within the beta-amyloid domain, incorrect beta-APP isoform expression in 
      fibroblasts or neuronal cells, or excess amounts of beta-APP all led to increases 
      in beta-amyloid production. Aberrant beta-APP appears to be diverted from the 
      secretory pathway and then degraded to beta-amyloid.
FAU - Zhong, Z
AU  - Zhong Z
AD  - Scios Nova Inc., Mountain View, California 94043.
FAU - Quon, D
AU  - Quon D
FAU - Higgins, L S
AU  - Higgins LS
FAU - Higaki, J
AU  - Higaki J
FAU - Cordell, B
AU  - Cordell B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sulfur Radioisotopes)
RN  - AE28F7PNPL (Methionine)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amyloid beta-Peptides/*biosynthesis/isolation & purification
MH  - Amyloid beta-Protein Precursor/*biosynthesis/isolation & purification/metabolism
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cysteine/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Kinetics
MH  - Methionine/metabolism
MH  - Models, Biological
MH  - Molecular Weight
MH  - Neuroblastoma
MH  - *Protein Processing, Post-Translational
MH  - Recombinant Proteins/biosynthesis/isolation & purification/metabolism
MH  - Sulfur Radioisotopes
MH  - Tumor Cells, Cultured
EDAT- 1994/04/22 00:00
MHDA- 1994/04/22 00:01
CRDT- 1994/04/22 00:00
PHST- 1994/04/22 00:00 [pubmed]
PHST- 1994/04/22 00:01 [medline]
PHST- 1994/04/22 00:00 [entrez]
AID - S0021-9258(17)32698-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1994 Apr 22;269(16):12179-84.