PMID- 8166435
OWN - NLM
STAT- MEDLINE
DCOM- 19940526
LR  - 20111206
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 14
IP  - 1A
DP  - 1994 Jan-Feb
TI  - Chromosome painting and quantitative karyotyping of colon adenocarcinoma cell 
      lines, DLD-1 and HCT-15.
PG  - 109-12
AB  - Chromosome painting by fluorescence in situ hybridization (FISH) was used to 
      examine abnormalities identified by G-banding in colon cancer lines, DLD-1 (ATCC 
      CCL 221) and HCT-15 (CCL 225). DNA libraries from chromosomes comprising these 
      abnormalities (i.e., N2, N8, N11, N16, N17 and N20) were used to prepare paint 
      probes by PCR amplification. Of these paint probes, N2 and N8 exhibited 
      additional chromosome-specific hybridization signals on centromeres that were 
      also useful as a marker for chromosome identification. Results from FISH-painting 
      and G-band analysis were consistent and permitted our quantitative analysis on 
      karyotype evolution in vitro. In DLD-1, predominant cells having trisomic N20 in 
      early passages were replaced by others with disomic N20 in late passages 
      resulting in the trisomic 2p13-23 segment as the only deviation from the diploid 
      content. In HCT-15, predominant cells having t(16;16) and double Y chromosome 
      copies in early passages were replaced by those bearing the paired N16 and single 
      Y chromosome in later passages. Thus cultures changed from the predominant 
      hyperdiploidy to the sole pseudodiploidy with increased number of normal 
      chromosomes.
FAU - Chen, T R
AU  - Chen TR
AD  - American Type Culture Collection, Rockville, MD 20852.
FAU - Nierman, W C
AU  - Nierman WC
LA  - eng
GR  - N01-CB-71014/CB/NCI NIH HHS/United States
GR  - N01-PR-9-2105/PR/OCPHP CDC HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Chromosome Banding
MH  - *Chromosomes, Human
MH  - Clone Cells
MH  - Colonic Neoplasms/*genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mosaicism
MH  - Tumor Cells, Cultured
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1994 Jan-Feb;14(1A):109-12.