PMID- 8166447
OWN - NLM
STAT- MEDLINE
DCOM- 19940526
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 14
IP  - 1A
DP  - 1994 Jan-Feb
TI  - Comparative interphase cytogenetics using FISH on human ovarian carcinomas.
PG  - 183-8
AB  - Analyses using fluorescence in situ hybridization (FISH) were performed on 
      cytogenetic slides of 25 human ovarian carcinomas. Biotinylated alphoid DNA 
      probes were used for the monocolor and bicolor detection of chromosomes #1, #7, 
      #8, #11, #12, #17 and #20 in interphase nuclei of the target cell material. 
      Specific loss of chromosomes #17 and #20 was the most significant finding in 
      aberrant cell populations of the tumors, as was gain of chromosomes #7, #1, #8 
      and #11. By the use of probe combinations, the presence of combined gains and 
      losses of several chromosomes within the same cell subpopulations could be shown 
      in a series of tumors, while in others the significant numerical chromosome 
      abnormalities found characterized various different cell populations. In summary, 
      FISH could be shown to be a powerful tool of interphase cytogenetics, provided 
      that its limitations are considered conscientiously.
FAU - Liehr, T
AU  - Liehr T
AD  - Institute of Human Genetics, University of Erlangen-Nurnberg Schwabachanlage, 
      Germany.
FAU - Stubinger, A
AU  - Stubinger A
FAU - Thoma, K
AU  - Thoma K
FAU - Tulusan, H A
AU  - Tulusan HA
FAU - Gebhart, E
AU  - Gebhart E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosomes, Human
MH  - DNA Probes
MH  - Female
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*physiology
MH  - Middle Aged
MH  - Ovarian Neoplasms/*genetics/pathology
MH  - Ploidies
MH  - Proto-Oncogenes
MH  - Signal Processing, Computer-Assisted
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1994 Jan-Feb;14(1A):183-8.