PMID- 8166944
OWN - NLM
STAT- MEDLINE
DCOM- 19940601
LR  - 20190920
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 31
IP  - 1
DP  - 1994 Jan
TI  - Flow cytometry analysis of lymphocyte subpopulations in peritoneal fluid of women 
      with endometriosis.
PG  - 25-31
AB  - PROBLEM: We investigated the lymphocyte subpopulations in peripheral blood (PB) 
      and peritoneal fluid (PF) of women with and without endometriosis to evaluate if 
      the decreased natural killer (NK)-mediated cytotoxicity in women with 
      endometriosis was due to a quantitative defect or not. METHOD: The PB and PF 
      mononuclear cells of 59 women undergoing a diagnostic laparoscopy for pain and/or 
      infertility were analyzed by flow cytometry. RESULTS: The number and 
      concentration of PF mononuclear cells (MC) was increased in women with 
      endometriosis compared to women without endometriosis. The monocyte/macrophage 
      marker (CD14) was expressed on 70.3 and 66.9% of PFMC of women with and without 
      endometriosis, respectively. The CD4/CD8 ratio was inverted in the PF, and this 
      was more pronounced in women with endometriosis. In the PF of women with 
      endometriosis, 41.3% of the lymphocytes were CD8 positive, compared to 34.3% in 
      women without endometriosis. The percentage of NK positive lymphocytes in PF, 
      using three different monoclonal antibodies directed against NK cell markers 
      (CD57, CD16, and CD56) were not different between women with and without 
      endometriosis. In women with endometriosis, 12.7, 9.5, and 28.8% of lymphocytes 
      were CD57, CD16, and CD56 positive, respectively. CONCLUSION: PFMC consisted 
      mainly of phagocytic and human leukocyte antigen (HLA)-restricted or HLA 
      unrestricted cytotoxic cells capable of reacting to various antigens entering the 
      cavity from the lower genital tractus. Furthermore, the decreased NK activity 
      reported in PB and PF of women with endometriosis was not likely to be caused by 
      a quantitative defect, since the percentage of NK positive lymphocytes was not 
      different between women with and without endometriosis.
FAU - Oosterlynck, D J
AU  - Oosterlynck DJ
AD  - Department of Obstetrics and Gynecology, University of Leuven, Belgium.
FAU - Meuleman, C
AU  - Meuleman C
FAU - Lacquet, F A
AU  - Lacquet FA
FAU - Waer, M
AU  - Waer M
FAU - Koninckx, P R
AU  - Koninckx PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
SB  - IM
MH  - Ascitic Fluid/immunology
MH  - Endometriosis/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Killer Cells, Natural
MH  - Leukocyte Count
MH  - *Lymphocyte Subsets
MH  - Phagocytes
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1111/j.1600-0897.1994.tb00843.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1994 Jan;31(1):25-31. doi: 
      10.1111/j.1600-0897.1994.tb00843.x.