PMID- 8168143
OWN - NLM
STAT- MEDLINE
DCOM- 19940602
LR  - 20131121
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 155
IP  - 1
DP  - 1994 Apr 15
TI  - Alteration of cell cycle kinetics and immunoglobulin gene transcription as the 
      result of multiple agonist stimulation of murine B cells.
PG  - 156-68
AB  - Several laboratories have established that anti-IgM can inhibit polyclonal B cell 
      activation by LPS or LPS/DxS. The use of intact anti-IgM results in an inhibition 
      of both proliferation and differentiation, whereas F(ab')2 fragments inhibit only 
      differentiation. Since signal transduction by both alpha-Ig's (intact and F(ab')2 
      fragments) is known to be mediated by PIP2 hydrolysis, we have investigated the 
      effects of A23187 and PMA on LPS/DxS activation of splenic B cells. These agents 
      mimic the second messengers generated as the results of PIP2 hydrolysis. As with 
      intact alpha-IgM, either agent in conjunction with LPS/DxS resulted in an 
      inhibition of proliferation as assessed by [3H]thymidine uptake. However, when 
      proliferation was assessed by acridine orange (AO) staining and flow cytometric 
      analysis, cells were observed to have entered cell cycle. This disparity between 
      AO staining and proliferation was resolved by using BrDu/Hoechst quenching 
      analysis and revealed a delay in cell cycle transit time as the result of 
      multiple agent stimulation. Since both anti-IgM's result in the inhibition of 
      differentiation, we also investigated the effects of these agents on 
      differentiation normally observed with LPS/DxS alone activation of B cells. 
      A23187 and PMA, either alone or in combination, were observed to result in a 
      decrease in mRNA-encoding mu immunoglobulin of the 2.4-kb mRNA for secreted IgM.
FAU - Jones, T L
AU  - Jones TL
AD  - Harry S. Truman Memorial VA Hospital, Columbia, Missouri.
FAU - Barnett, L
AU  - Barnett L
FAU - Lafrenz, D
AU  - Lafrenz D
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Immunoglobulin M)
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*drug effects/immunology
MH  - Calcimycin/pharmacology
MH  - Cell Cycle/*drug effects
MH  - Cell Differentiation/*drug effects
MH  - Dextran Sulfate/pharmacology
MH  - Female
MH  - Genes, Immunoglobulin/*drug effects
MH  - Immunoglobulin M/metabolism
MH  - Immunoglobulin mu-Chains/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Messenger/analysis
MH  - Spleen/cytology/immunology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription, Genetic/*drug effects
EDAT- 1994/04/15 00:00
MHDA- 1994/04/15 00:01
CRDT- 1994/04/15 00:00
PHST- 1994/04/15 00:00 [pubmed]
PHST- 1994/04/15 00:01 [medline]
PHST- 1994/04/15 00:00 [entrez]
AID - S0008-8749(84)71109-9 [pii]
AID - 10.1006/cimm.1994.1109 [doi]
PST - ppublish
SO  - Cell Immunol. 1994 Apr 15;155(1):156-68. doi: 10.1006/cimm.1994.1109.