PMID- 8174548
OWN - NLM
STAT- MEDLINE
DCOM- 19940609
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 221
IP  - 2
DP  - 1994 Apr 15
TI  - Purification and characterisation of a brain-derived neurotrophic 
      factor/neurotrophin-3 (BDNF/NT-3) heterodimer.
PG  - 677-85
AB  - The neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF), and neurotrophin-3 (NT-3) are known to exist in solution as 
      non-covalently linked homodimeric proteins. The recent elucidation of the crystal 
      structure of the NGF homodimer, as well as the conservation of structural motifs 
      in the neurotrophins, raised the possibility that neurotrophin heterodimers might 
      also occur. The formation of BDNF/NT-3 heterodimers was explored using a vaccinia 
      virus expression system. Upon co-infection of cells with viruses expressing BDNF 
      and NT-3, we could identify a BDNF/NT-3 heterodimer as a biosynthetic product and 
      separate it from the BDNF and NT-3 homodimers. We could also show that the 
      BDNF/NT-3 heterodimers can be formed irrespective of wild-type or exchanged 
      prosequences, indicating that prosequence specificity does not influence dimer 
      formation. In all neuronal survival assays that were used, the BDNF/NT-3 
      heterodimer was shown to be 10-fold less active compared with a mixture of BDNF 
      and NT-3 homodimers. This lower specific activity was also measured in a neuronal 
      population co-expressing receptors for BDNF and NT-3. The low biological activity 
      of the heterodimer observed with neurons was not paralleled by a reduced ability 
      of the heterodimer to interact with trkB or trkC receptors, as assessed by the 
      induction of tyrosine phosphorylation of these receptors expressed by fibroblast 
      cell lines.
FAU - Jungbluth, S
AU  - Jungbluth S
AD  - Max-Planck Institute for Psychiatry, Department of Neurobiochemistry, 
      Martinsried, Germany.
FAU - Bailey, K
AU  - Bailey K
FAU - Barde, Y A
AU  - Barde YA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Polymers)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chick Embryo
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nerve Growth Factors/*chemistry/isolation & purification/metabolism
MH  - Nerve Tissue Proteins/*chemistry/isolation & purification/metabolism
MH  - Neurons/cytology/drug effects
MH  - Neurotrophin 3
MH  - Polymers
MH  - Rabbits
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptor, trkC
MH  - Receptors, Growth Factor/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Vaccinia virus/genetics/metabolism
EDAT- 1994/04/15 00:00
MHDA- 1994/04/15 00:01
CRDT- 1994/04/15 00:00
PHST- 1994/04/15 00:00 [pubmed]
PHST- 1994/04/15 00:01 [medline]
PHST- 1994/04/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1994.tb18780.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1994 Apr 15;221(2):677-85. doi: 
      10.1111/j.1432-1033.1994.tb18780.x.