PMID- 8175016
OWN - NLM
STAT- MEDLINE
DCOM- 19940603
LR  - 20061115
IS  - 0090-8258 (Print)
IS  - 0090-8258 (Linking)
VI  - 53
IP  - 1
DP  - 1994 Apr
TI  - Transforming growth factor alpha localization and role in surface epithelium of 
      normal human ovaries and in ovarian carcinoma cells.
PG  - 17-23
AB  - Transforming growth factor alpha (TGF alpha) has been localized by 
      immunohistochemistry in the ovarian surface epithelial (OSE) cells of sections 
      from normal human ovaries and in epithelial cells of surface crypts. An ovarian 
      cancer cell line (HEY) derived from the surface epithelium of a human ovary also 
      exhibited intense staining for the TGF alpha peptide. Using Northern analysis, 
      HEY cells were shown to express a 4.5-kb transcript of TGF alpha, indicating that 
      the TGF alpha peptide was synthesized by these cells and not taken up from the 
      serum in the culture medium and sequestered by the cells. This was confirmed 
      using a radioimmunoassay, which showed that HEY cells in culture secrete TGF 
      alpha peptide, both as a soluble (0.12 +/- 0.02 ng/mg protein) and as a 
      membrane-anchored (0.06 +/- 0.006 ng/mg protein) form. In both normal OSE cells 
      and HEY cells, TGF alpha acted as a growth promoter: TGF alpha significantly 
      stimulated [3H]thymidine incorporation into DNA of both primary cultures of 
      normal OSE cells (2.7-fold) and of HEY cells (2-fold). This study provides the 
      first demonstration of TGF alpha immunostaining in normal surface epithelial 
      cells and in HEY cells, and suggests that TGF alpha, localized in normal and 
      transformed OSE, is an autocrine growth promoter for these cells.
FAU - Jindal, S K
AU  - Jindal SK
AD  - Banting & Best Department of Medical Research, University of Toronto, Ontario, 
      Canada.
FAU - Snoey, D M
AU  - Snoey DM
FAU - Lobb, D K
AU  - Lobb DK
FAU - Dorrington, J H
AU  - Dorrington JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gynecol Oncol
JT  - Gynecologic oncology
JID - 0365304
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Cell Division/physiology
MH  - Cell Transformation, Neoplastic/pathology
MH  - Cells, Cultured
MH  - Epithelium/physiology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Ovarian Neoplasms/*chemistry/pathology/*physiopathology
MH  - Ovary/*chemistry/cytology/*physiology
MH  - Radioimmunoassay
MH  - Transforming Growth Factor alpha/*analysis/*physiology
MH  - Tumor Cells, Cultured
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - S0090-8258(84)71080-8 [pii]
AID - 10.1006/gyno.1994.1080 [doi]
PST - ppublish
SO  - Gynecol Oncol. 1994 Apr;53(1):17-23. doi: 10.1006/gyno.1994.1080.