PMID- 8177394
OWN - NLM
STAT- MEDLINE
DCOM- 19940608
LR  - 20190512
IS  - 0148-396X (Print)
IS  - 0148-396X (Linking)
VI  - 34
IP  - 2
DP  - 1994 Feb
TI  - The role of brain-derived neurotrophic factor in transient forebrain ischemia in 
      the rat brain.
PG  - 323-31; discussion 331
AB  - Brain-derived neurotrophic factor (BDNF) may play a role in the pathophysiology 
      of neuronal cell death after cerebral ischemia. We investigated alterations in 
      BDNF gene expression and the effect of BDNF on neuronal death after transient 
      forebrain ischemia in the rat brain. Transient forebrain ischemia was induced by 
      occlusion of the bilateral common carotid arteries and by producing systemic 
      hypotension for 8 minutes. The alterations in the BDNF messenger ribonucleic acid 
      content in the hippocampus and the cerebral cortex were examined by Northern blot 
      analysis, using a phosphorus-32-labeled mouse BDNF complementary deoxyribonucleic 
      acid probe. Recombinant Chinese hamster ovary cells with BDNF-secreting capacity 
      were established by expression vector transfection with BDNF complementary 
      deoxyribonucleic acid. The effect of BDNF on neuronal death in the hippocampal 
      CA1 region after ischemia was then examined by using a continuous 
      intraventricular infusion of 200 microliters of normal (Group II, n = 6) or 
      30-times concentrated recombinant Chinese hamster ovary cell culture medium 
      containing BDNF (Group IV, n = 6). Normal (Group I, n = 6) or 30-times 
      concentrated (Group III, n = 6) Chinese hamster ovary cell culture medium, not 
      including BDNF complementary deoxyribonucleic acid, was infused into the same 
      ischemic brains, which served as controls.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Tsukahara, T
AU  - Tsukahara T
AD  - Department of Cerebrovascular Surgery, National Cardiovascular Center, Kyoto, 
      Japan.
FAU - Yonekawa, Y
AU  - Yonekawa Y
FAU - Tanaka, K
AU  - Tanaka K
FAU - Ohara, O
AU  - Ohara O
FAU - Wantanabe, S
AU  - Wantanabe S
FAU - Kimura, T
AU  - Kimura T
FAU - Nishijima, T
AU  - Nishijima T
FAU - Taniguchi, T
AU  - Taniguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurosurgery
JT  - Neurosurgery
JID - 7802914
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Survival/physiology
MH  - Gene Expression Regulation/physiology
MH  - Hippocampus/blood supply/pathology
MH  - Ischemic Attack, Transient/pathology/*physiopathology
MH  - Male
MH  - Nerve Tissue Proteins/genetics/*physiology
MH  - Neurons/pathology/physiology
MH  - Prosencephalon/*blood supply/pathology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1227/00006123-199402000-00016 [doi]
PST - ppublish
SO  - Neurosurgery. 1994 Feb;34(2):323-31; discussion 331. doi: 
      10.1227/00006123-199402000-00016.