PMID- 8178559
OWN - NLM
STAT- MEDLINE
DCOM- 19940606
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 12
IP  - 4
DP  - 1994 Mar
TI  - Recombinant derivative of a naturally occurring non-toxinogenic Vibrio cholerae 
      01 expressing the B subunit of cholera toxin: a potential oral vaccine strain.
PG  - 359-64
AB  - A clinical isolate of Vibrio cholerae 01 was identified which did not possess the 
      heat-labile (CT), the heat-stable (ST) or the zonula occludens (Zot) toxin genes. 
      Rabbit ileal loop assays showed that no other CT-like toxin was produced by this 
      strain. The partly deleted cholera toxin gene which carries the intact gene for 
      the B subunit was cloned and the recombinant plasmid, pURD110, was introduced 
      into this non-toxinogenic natural human isolate. The transformed cells (strain 
      URD2) secreted the B subunit gene product which competed with the holotoxin 
      secreted by the hypertoxinogenic strain 569B of V. cholerae for the GM1 
      ganglioside binding sites in vivo. This strain can colonize the rabbit intestine 
      as detected by the removable intestinal tie adult rabbit diarrhoea (RITARD) 
      model. This construct has an advantage over other live oral attenuated V. 
      cholerae strains used as vaccines in that the latter strains were made 
      non-toxinogenic by only deleting part of the gene coding for the A subunit of 
      cholera toxin while the strain described here is naturally non-toxinogenic.
FAU - Dasgupta, U
AU  - Dasgupta U
AD  - Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
FAU - Bhadra, R K
AU  - Bhadra RK
FAU - Panda, D K
AU  - Panda DK
FAU - Deb, A
AU  - Deb A
FAU - Das, J
AU  - Das J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Cholera Vaccines)
RN  - 0 (Vaccines, Synthetic)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Cholera Toxin/*immunology/toxicity
MH  - Cholera Vaccines/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Intestines/microbiology
MH  - Rabbits
MH  - Vaccines, Synthetic/immunology
MH  - Vibrio cholerae/*immunology/pathogenicity
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 0264-410X(94)90101-5 [pii]
AID - 10.1016/0264-410x(94)90101-5 [doi]
PST - ppublish
SO  - Vaccine. 1994 Mar;12(4):359-64. doi: 10.1016/0264-410x(94)90101-5.