PMID- 8180392
OWN - NLM
STAT- MEDLINE
DCOM- 19940614
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 83
IP  - 10
DP  - 1994 May 15
TI  - Detection of 14q32 translocations in B-cell malignancies by in situ hybridization 
      with yeast artificial chromosome clones containing the human IgH gene locus.
PG  - 2962-9
AB  - Partner sites of 14q32 translocations found in B-cell malignancies were detected 
      by fluorescence in situ hybridization (FISH) using yeast artificial chromosome 
      (YAC) clones, Y20 and Y6, containing the human Ig heavy chain (IgH) gene locus. 
      Y20 spans a 160-kb upstream and 40-kb downstream region of the JH segments on 
      chromosome band 14q32.33. Y6 is 300-kb upstream of Y20, and spans a further 
      320-kb telomeric region. The human DNA sequences amplified by Alu polymerase 
      chain reaction of the YAC clones were used as probes for FISH to study six 
      patients with non-Hodgkin's lymphoma (NHL), one patient with acute lymphoblastic 
      leukemia, and one cell line FR4 established from a plasmacytoma. Three telomeric 
      YAC clones each specific for 3q, 8q, and 18q were also used to further 
      characterize 14q32 translocations. The IgH YACs were successfully applied to 
      detect cytogenetically invisible subtelomeric translocation of the IgH gene locus 
      to each partner site in t(14;18), t(8;14), and t(14;19), and to identify t(3;14) 
      (q27;q32.33) in three patients with 14q32 translocation of unknown origin. 
      Furthermore, complex translocations involving more than three chromosomes were 
      detected in an NHL patient with t(8;14), and t(3;12), and in the FR4 with 
      der(14)t(8;14), der(8)dic(1;8), and del(1)(q21). The technique would be a useful 
      tool in elucidating the mechanisms of a 14q32 translocation in B-cell 
      malignancies.
FAU - Taniwaki, M
AU  - Taniwaki M
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Matsuda, F
AU  - Matsuda F
FAU - Jauch, A
AU  - Jauch A
FAU - Nishida, K
AU  - Nishida K
FAU - Takashima, T
AU  - Takashima T
FAU - Tagawa, S
AU  - Tagawa S
FAU - Sugiyama, H
AU  - Sugiyama H
FAU - Misawa, S
AU  - Misawa S
FAU - Abe, T
AU  - Abe T
FAU - Kashima, K
AU  - Kashima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Aged
MH  - Child
MH  - Chromosome Mapping
MH  - *Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 14
MH  - Female
MH  - *Genes, Immunoglobulin
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 1994/05/15 00:00
MHDA- 1994/05/15 00:01
CRDT- 1994/05/15 00:00
PHST- 1994/05/15 00:00 [pubmed]
PHST- 1994/05/15 00:01 [medline]
PHST- 1994/05/15 00:00 [entrez]
AID - S0006-4971(20)69214-X [pii]
PST - ppublish
SO  - Blood. 1994 May 15;83(10):2962-9.