PMID- 8180398
OWN - NLM
STAT- MEDLINE
DCOM- 19940614
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 83
IP  - 10
DP  - 1994 May 15
TI  - Adhesion of sickle cells to vascular endothelium is critically dependent on 
      changes in density and shape of the cells.
PG  - 3006-17
AB  - Experiments were performed to explore whether adhesion differences among 
      different density sickle (SS) cells are mainly caused by deformability 
      characteristics attendant to density variations, which could affect their surface 
      contacts with the endothelium, or caused by any changes in their membrane surface 
      (eg, receptors). Also, what is the contribution of morphologic attributes of SS 
      cells in adhesive and obstructive events? To resolve these issues, controlled 
      modifications of cell density were performed using Nystatin and sucrose. This 
      allowed elevation of the mean corpuscular hemoglobin concentration (MCHC) of 
      light-density SS2 discocytes to that of SS4 native dense cells, as well as its 
      normalization in both native and artificially dehydrated cells. Hemodynamic and 
      adhesive characteristics of these individual SS cell populations and their 
      defined mixtures were investigated in the ex vivo mesocecum vasculature. 
      Dehydrated (high MCHC) SS2 discocytes, alone or with SS2 control cells, caused a 
      higher peripheral resistance but no persistent microvascular blockage. 
      Nevertheless, dehydrated SS2 discocytes resulted in a significantly decreased 
      adhesion in venules (adhesion sites). Rehydration of these high MCHC discocytes 
      completely restored their adhesivity, similar to that of SS2 control cells. In 
      contrast, irreversibly sickled cell (ISC)-rich SS4 dense cell class (dense 
      discocytes and 66% to 72% ISCs) was less adherent than dehydrated SS2 discocytes, 
      but caused a persistent blockage of small diameter postcapillary venules when 
      infused with SS2 control or dehydrated discocytes. In the areas of adhesion, SS4 
      dense discocytes outnumbered ISCs by 4 to 1, demonstrating that ISCs, because of 
      their shape characteristics, are minimally adherent but play a distinct role in 
      postcapillary obstruction. When MCHC (density) of SS4 cells was decreased by 
      rehydration, it resulted in an almost complete reversal of their hemodynamic and 
      adhesive behavior, confirming a profound influence of cell density. These 
      findings demonstrate for the first time that, under shear flow, adhesion of 
      different density SS cells to the endothelium is dependent on the inverse of cell 
      density rather than any changes in their adhesion potential. Finally, the 
      trapping of ISC-rich native dense cells, but not of dehydrated discocytes, in the 
      areas of adhesion shows a distinct contribution of ISC in adhesion-initiated 
      vasoocclusion.
FAU - Kaul, D K
AU  - Kaul DK
AD  - Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
FAU - Chen, D
AU  - Chen D
FAU - Zhan, J
AU  - Zhan J
LA  - eng
GR  - HL 37212/HL/NHLBI NIH HHS/United States
GR  - HL 45931/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Adult
MH  - Anemia, Sickle Cell/*blood
MH  - Animals
MH  - Cell Adhesion
MH  - Endothelium, Vascular/*cytology/physiology
MH  - Erythrocyte Deformability
MH  - Erythrocyte Indices
MH  - Erythrocytes/*physiology
MH  - Hemodynamics
MH  - Humans
MH  - Perfusion
MH  - Rats
EDAT- 1994/05/15 00:00
MHDA- 1994/05/15 00:01
CRDT- 1994/05/15 00:00
PHST- 1994/05/15 00:00 [pubmed]
PHST- 1994/05/15 00:01 [medline]
PHST- 1994/05/15 00:00 [entrez]
AID - S0006-4971(20)69220-5 [pii]
PST - ppublish
SO  - Blood. 1994 May 15;83(10):3006-17.