PMID- 8180985
OWN - NLM
STAT- MEDLINE
DCOM- 19940616
LR  - 20190813
IS  - 0008-6215 (Print)
IS  - 0008-6215 (Linking)
VI  - 254
DP  - 1994 Feb 17
TI  - Preparation and properties of metabolically 3H- or 13C-labeled 
      (1-->3)-beta-D-glucan, SSG, from Sclerotinia sclerotiorum IFO 9395.
PG  - 213-9
AB  - Metabolically labeled (1-->3)-beta-D-glucan (SSG) obtained from the culture 
      filtrate of Sclerotinia sclerotiorum IFO 9395, with the radio (3H) or the stable 
      isotope (13C) was prepared. The specific radioactivity of 3H-SSG was increased in 
      accordance with the amount of D-[3H]glucose added, and was 15-20 kBq/mg SSG when 
      222 kBq/mL of D-[3H]glucose was added. Physicochemical analyses of 3H-SSG 
      suggested a structural similarity between SSG and 3H-SSG. 13C NMR spectra of 
      13C-labeled SSG (13C-SSG) revealed that a strong signal of the 13C-nucleus was 
      observed at the C-1 or C-2 position when D-[1-13C]glucose or D-[2-13C]glucose was 
      used, respectively, suggesting that most of the glucose residues in SSG were 
      directly taken up from the medium. In addition, part of the 13C-nucleus was 
      incorporated into the SSG molecule at all carbon atoms after metabolic 
      degradation and reconstruction of the glucose molecule. Analyses of the culture 
      filtrate and the mycelium of the fungus suggested that part of the glucose was 
      also metabolized to trehalose and mannitol.
FAU - Suda, M
AU  - Suda M
AD  - Laboratory of Immunopharmacology of Microbial Products, Tokyo College of 
      Pharmacy, Japan.
FAU - Ohno, N
AU  - Ohno N
FAU - Adachi, Y
AU  - Adachi Y
FAU - Yadomae, T
AU  - Yadomae T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Carbohydr Res
JT  - Carbohydrate research
JID - 0043535
RN  - 0 (Carbon Isotopes)
RN  - 0 (Glucans)
RN  - 0 (beta-Glucans)
RN  - 10028-17-8 (Tritium)
RN  - 9051-97-2 (beta-1,3-glucan)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Ascomycota/chemistry/growth & development/*metabolism
MH  - Carbohydrate Conformation
MH  - Carbon Isotopes
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - Glucans/*biosynthesis/*chemistry/isolation & purification
MH  - Glucose/metabolism
MH  - Isotope Labeling
MH  - Magnetic Resonance Spectroscopy
MH  - Tritium
MH  - *beta-Glucans
EDAT- 1994/02/17 00:00
MHDA- 1994/02/17 00:01
CRDT- 1994/02/17 00:00
PHST- 1994/02/17 00:00 [pubmed]
PHST- 1994/02/17 00:01 [medline]
PHST- 1994/02/17 00:00 [entrez]
AID - 0008-6215(94)84254-X [pii]
AID - 10.1016/0008-6215(94)84254-x [doi]
PST - ppublish
SO  - Carbohydr Res. 1994 Feb 17;254:213-9. doi: 10.1016/0008-6215(94)84254-x.