PMID- 8183853
OWN - NLM
STAT- MEDLINE
DCOM- 19940616
LR  - 20190904
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 14
IP  - 2
DP  - 1994 Feb
TI  - Evaluation of X, Y, 18, and 13/21 alpha satellite DNA probes for interphase 
      cytogenetic analysis of uncultured amniocytes by fluorescence in situ 
      hybridization.
PG  - 79-86
AB  - The major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, 
      and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid 
      analysis of chromosome copy number in interphase cells. This prospective study 
      evaluated the use of four commercially available centromeric DNA probes (DXZ1, 
      DYZ1, D18Z1, and D13Z1/D21Z1) for direct analysis of uncultured amniocytes. One 
      hundred and sixteen amniotic fluid samples were analysed by FISH and standard 
      cytogenetics. This evaluation demonstrated that FISH with X, Y, and 18 alpha 
      satellite DNA probes could accurately and rapidly detect aneuploidies involving 
      these chromosomes and could be used in any prenatal clinical laboratory. In 
      contrast, the 13/21 alpha satellite DNA probe hybridizing both chromosomes 13 and 
      21 was unreliable for prenatal diagnosis in uncultured amniocytes.
FAU - Cacheux, V
AU  - Cacheux V
AD  - Unite de Cytogenetique, Hopital Robert Debre, Paris, France.
FAU - Tachdjian, G
AU  - Tachdjian G
FAU - Druart, L
AU  - Druart L
FAU - Oury, J F
AU  - Oury JF
FAU - Serero, S
AU  - Serero S
FAU - Blot, P
AU  - Blot P
FAU - Nessmann, C
AU  - Nessmann C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - *Aneuploidy
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 21
MH  - *DNA Probes
MH  - DNA, Satellite
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Male
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Prospective Studies
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1002/pd.1970140202 [doi]
PST - ppublish
SO  - Prenat Diagn. 1994 Feb;14(2):79-86. doi: 10.1002/pd.1970140202.