PMID- 8185829
OWN - NLM
STAT- MEDLINE
DCOM- 19940622
LR  - 20191023
IS  - 0899-1987 (Print)
IS  - 0899-1987 (Linking)
VI  - 10
IP  - 1
DP  - 1994 May
TI  - Absence of p53 mutations and various frequencies of Ki-ras exon 1 mutations in 
      rat hepatic tumors induced by different carcinogens.
PG  - 45-51
AB  - Mutations of p53 and Ki-ras exon 1 were investigated in rat hepatic lesions 
      induced by four kinds of hepatocarcinogenic protocols: continuous feeding of 
      3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB), daily intraperitoneal injection 
      of aflatoxin B1 (AFB1), and the Solt and Farber regimen (Nature 236:701-703, 
      1976), in which diethylnitrosamine (DEN) or nitrosomethylurea (NMU) was used as 
      initiating agents. DNA from microdissected tissue sections was amplified by the 
      polymerase chain reaction (PCR) directly using primers for p53 exons 5-8 and 
      Ki-ras exon 1 and analyzed for mutations by denaturing gradient gel 
      electrophoresis (DGGE) or constant denaturant gel electrophoresis (CDGE). One or 
      both of the p53 PCR primers were located within introns to prevent amplifying the 
      p53 processed pseudogenes. In a total of 59 hepatocellular carcinomas (HCCs), no 
      p53 aberrations were detected, indicating that p53 mutations are not very 
      important in rat hepatic carcinogenesis. On the other hand, Ki-ras codon 12 
      mutations were found at low frequency in HCCs, hyperplastic foci, and 
      cholangiofibroses induced by 3'-Me-DAB and by AFB1 but not in the lesions induced 
      by the Solt and Farber regimen. Although Ki-ras codon 12 mutations are generally 
      infrequent in rat hepatic tumors, their incidence thus appears to vary depending 
      on the carcinogen used for their induction.
FAU - Tokusashi, Y
AU  - Tokusashi Y
AD  - Department of Pathology, Asahikawa Medical College, Japan.
FAU - Fukuda, I
AU  - Fukuda I
FAU - Ogawa, K
AU  - Ogawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Carcinog
JT  - Molecular carcinogenesis
JID - 8811105
RN  - 0 (Carcinogens)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
SB  - IM
GS  - Ki-ras
GS  - p53
MH  - Animals
MH  - Base Sequence
MH  - Carcinogens
MH  - DNA Primers/chemistry
MH  - DNA, Neoplasm/genetics
MH  - *Genes, p53
MH  - *Genes, ras
MH  - Liver Neoplasms/*genetics
MH  - Liver Neoplasms, Experimental/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Point Mutation
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1002/mc.2940100108 [doi]
PST - ppublish
SO  - Mol Carcinog. 1994 May;10(1):45-51. doi: 10.1002/mc.2940100108.