PMID- 8185841
OWN - NLM
STAT- MEDLINE
DCOM- 19940621
LR  - 20180216
IS  - 1015-3837 (Print)
IS  - 1015-3837 (Linking)
VI  - 9
IP  - 2
DP  - 1994 Mar-Apr
TI  - Prenatal diagnosis by fluorescence in situ hybridization on chorionic villus 
      cells: nonsignificance of maternal cell contamination.
PG  - 73-6
AB  - We assessed the effect of maternal cell contamination on the sensitivity of 
      prenatal diagnosis by fluorescence in situ hybridization (FISH) on overnight 
      attached mesenchymal chorionic villus cells. Double targets FISHs with X and Y 
      chromosome-specific probes were performed on parallel samples from the same 
      pregnancies: (1) samples of assumed fetal tissue retained during dissection, and 
      (2) samples of suspected maternal tissue discarded during dissection. In the 
      karyotypically male samples the tissue retained during dissection contained 0-3% 
      nuclei with two X-specific signals each. In the tissue discarded from the 
      karyotypically male samples 0-50% of the nuclei had two X-specific signals each. 
      We conclude that given thorough dissection of chorionic villi, the sensitivity of 
      prenatal diagnosis of aneuploidies by FISH on overnight attached samples of this 
      tissue is not critically affected by maternal cell contamination.
FAU - Bryndorf, T
AU  - Bryndorf T
AD  - Department of Obstetrics and Gynaecology, Rigshospitalet, Copenhagen, Denmark.
FAU - Christensen, B
AU  - Christensen B
FAU - Xiang, Y
AU  - Xiang Y
FAU - Philip, J
AU  - Philip J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Fetal Diagn Ther
JT  - Fetal diagnosis and therapy
JID - 9107463
SB  - IM
MH  - *Chorionic Villi Sampling
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Pregnancy
MH  - Sensitivity and Specificity
MH  - Single-Blind Method
MH  - Specimen Handling
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 10.1159/000263910 [doi]
PST - ppublish
SO  - Fetal Diagn Ther. 1994 Mar-Apr;9(2):73-6. doi: 10.1159/000263910.